Human iggfrom serum

Manufactured by Merck Group

Sourced in United States

The Human IgG from serum is a laboratory product that contains immunoglobulin G (IgG) purified from human serum. IgG is the most abundant type of antibody found in the human body and plays a crucial role in the immune system's response to pathogens. This product is intended for use in scientific research and laboratory applications, where IgG may be required as a reference standard or for the development of immunoassays.

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Human serum

2 protocols using human iggfrom serum

Human IgG N-Glycan Analysis

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Human IgG
from serum, bovine
fetuin, 2-aminobenzamide (2-AB), 2-aminobenzoic acid (2-AA), 2-¹³[C₆]-aminobenzoic acid, iodoacetamide, dithiothreitol,
dimethyl sulfoxide (DMSO), methylamine hydrochloride, (7-azabenzotriazol-1-yloxy)trispyrrolidinophosphonium
hexafluorophosphate (PyAOP), 4-methylmorpholine, sodium hydroxide,
cellulose (medium fibrous), iodomethane, and 2,5-dihydroxybenzoic
acid (DHB) were purchased from Sigma (St. Louis, MO). N-Glycosidase
F (PNGase F) was obtained from New England Biolabs (Ipswich, MA).
The Viva Spin series of spin filters (10K and 30K MWCO) were purchased
from Sartorius Stedium Biotech (Aubagne, France).

Zhou H., Warren P.G., Froehlich J.W, & Lee R.S. (2014). Dual Modifications Strategy to Quantify Neutral and Sialylated N-Glycans Simultaneously by MALDI-MS. Analytical Chemistry, 86(13), 6277-6284.

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Quantification of Human IgG Antibodies in Serum and Liver

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To determine the human IgG‐based antibody concentrations in serum and hepatic tissue samples, 96‐well ELISA plates were precoated with an anti‐human IgG antibody (Fab specific; Sigma‒Aldrich, USA) and appropriately blocked. Serum or liver lysate samples were diluted and added to the reaction wells. Antibodies were detected with an HRP‐conjugated anti‐human kappa light chain secondary antibody (Invitrogen, USA), and a chromogenic substrate and stop solution was subsequently used. The antibody concentrations were quantified by using human IgG from serum (Sigma‒Aldrich, USA) with known concentrations as standards.
For the detection of serum ant‐HBs levels, recombinant HBsAg protein (2 µg mL⁻¹) was coated onto ELISA 96‐well plates. Serum samples were diluted 50‐fold and added to pre‐coated HBsAg. The presence of mouse anti‐HBs was detected using HRP‐conjugated goat anti‐mouse IgG (H+L) secondary antibody (ab6789, Abcam, UK). The following steps were performed according to the manufacturer's protocols.

Jiang Y., Chen X., Ye X., Wen C., Xu T., Yu C., Ning W., Wang G., Xiang X., Liu X., Wang Y., Chen Y., Liu X., Shi C., Liu C., Yuan Q., Chen Y., Zhang T., Luo W, & Xia N. (2024). A Dual‐domain Engineered Antibody for Efficient HBV Suppression and Immune Responses Restoration. Advanced Science, 11(15), 2305316.

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