Tetranor pgdm

AminoxyTMT sixplex Label Reagent Set was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Phospholipid mixtures were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Lipid mediator standards, 12-HETE, 20-HETE, TXB₂, PGE₂, arachidonic acid, tetranor-PGDM, and 20-carboxy-LTB₄ were obtained from Cayman Chemical (Ann Arbor, MI, USA). LPA (16:0), LPA (18:1), and PA (36:2) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). DMTMM and 4-methylmorpholine (NMM) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) was obtained from Fuji Film Wako Pure Chemical Co. (Osaka, Japan). Special grade reagents of ammonium bicarbonate, sodium chloride and 1-buthanol, and HPLC grade solvents of methanol, isopropanol and acetonitrile were purchased from Wako Pure Chemical Co. The authentic lipid standard stock solutions were 12-HETE (0.6 μg/mL), 20-HETE (0.6 μg/mL), TXB₂ (0.6 μg/mL), PGE₂ (0.6 μg/mL), AA (0.6 μg/mL), tetranor-PGDM (0.6 μg/mL), 20-carboxy-LTB₄ (0.6 μg/mL), LPA(16:0) (10 μM), LPA (18:1) (10 μM), PA (36:2) (5 μg/mL).