Dulbecco s modified eagle medium dmem high glucose medium

As shown in Figure 6, the single peptide of 99% purity was synthesized and bought from GL Biochem Ltd. (Shanghai, China), TSP; 3-isobutyl-1-methylxanthine (IBMX), dexamethasone, Insulin, LPS, and PI3K inhibitor (LY294002, abbreviated as LY) Purchased from Sigma Aldrich, St. Louis, MO, USA; Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium, calf serum (NBCS), fetal bovine serum (FBS), penicillin-streptomycin, and 0.25% trypsin were purchased from Gibco, USA; Glycerin Content Detection Kit was purchased from Nanjing Jiancheng Bioengineering Institute; Fluorescent Probe for Glucose Uptake and Transport (2-NBDG) Absorption Kit was purchased from American BioVision Company; anti-p-Akt (4060S), anti-Akt(9272S), anti-GLUT4 (2213), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, AB-P-R001) were purchased from Cell Signaling Technology.

Vero cells were a gift from Professor Wu Zhiwei of Nanjing University. Vero cells, Mouse leukemia cells of monocyte macrophage line (RAW264.7, CoBioer Biosciences Co., Ltd. Nanjing, China) and Human corneal endothelial cell line (B4G12, DSMZ; ACC-647, Shanghai GuanDao Biological Engineering Co., Ltd.) were cultured in Dulbecco’s modified Eagle medium (DMEM) high glucose medium (Gibco, United States), Human corneal epithelial cell line (HCEC, CRL-11135, HCE-2; ATCC, Manassas, VA) were cultured in DMEM/F12 medium (Gibco, United States), and human myeloid leukemia mononuclear cell line (THP-1, CoBioer Biosciences Co., Ltd. Nanjing, China) were cultured in RPMI Medium 1640 (Invitrogen, Carlsbad, CA, United States), with 10% (v/v) fetal calf serum, 100 U/ml penicillin, and 100 g/ml streptomycin (Gibco, United States) in a humidified 37°C 5% CO₂ incubator. HSV-1 strain McKrae was kindly provided by Dr. Pedram Hamrah of Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, United States, which was prepared as previously described (Jamali et al., 2020 (link); Liu et al., 2021 (link)). Viruses were propagated, and titers were determined by plaque assay on Vero cells (Knipe and Spang, 1982 (link)).

Polylactic acid (molecular weight: 300,000, Cat No. 1604000258) was purchased from Corbion (Netherlands). Gelatin (sourced from pigskin, type A, ~228 g bloom, viscosity (6.67%, 60°C): 3.8 mPas, Cat No. 1010583) was purchased from GELITA (United States, US). Hexafluoroisopropanol (HFIP, Analytical Pure) was purchased from Halocarbon Company (USA). Poly (lactic acid) and gelatin at a mass ratio of 7:3 were added to HFIP, stirred at room temperature for 12 h until completely dissolved, and the spinning solution was prepared to have a total concentration of 8% (w/v). Dulbecco's modified eagle medium (DMEM) high-glucose medium, fetal bovine serum, pancreatin, and phosphate buffer were purchased from Gibco (USA); Alamar Blue was purchased from Maibio (Shanghai, China); the CCK-8 kit was purchased from Dojindo (Japan). The live/dead cell viability staining kit was purchased from KeyGen Biotech (Nanjing, China).