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Anti tim23

Manufactured by Proteintech
Sourced in United States

Anti-Tim23 is a lab equipment product that is used to detect the Tim23 protein. Tim23 is a component of the translocase of the inner mitochondrial membrane (TIM23) complex, which is involved in the import and translocation of proteins into the mitochondria. Anti-Tim23 can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the localization and expression of Tim23 in different cell types or tissues.

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3 protocols using anti tim23

1

Antibody Characterization for Cell Biology

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Anti-Tim23 (1/1,000), anti-Tom20 (for western blots, 1/1,000, for immunofluorescence, 1/200), anti-Bcl2-L-13 (1/1,000) (Proteintech), anti-cytochrome c (1/1,000) (BD biosciences), anti-Bcl2-L-13 (1/500), anti-GAPDH (1/1,000), anti-Fis1 (1/1,000), anti-α-tubulin (1/1,000) (Abcam), anti-LC3B (for western blots, 1/1,000, for immunofluorescence, 1/100), anti-HA (for western blots, 1/1,000, for immunofluorescence, 1/200), anti-Drp1Ser637 (1/500), anti-Drp1Ser616 (1/1,000), anti-Mfn2 (1/1,000), anti-cleaved caspase 3 (1/1,000) (Cell Signaling Technology), anti-ATP synthase subunit beta (for western blots, 1/1,000, for immunofluorescence, 1/200) (Life Technologies), anti-Drp1 (for western blots, 1/1,000, for immunofluorescence, 1/100), anti-Opa1 (1/1,000) (BD Transduction Laboratories), anti-FLAG (for immunofluorescence, 1/200) (Sigma), anti-Mfn1 (1/1,000) (Abnova), anti-ubiquitin (for immunofluorescence, 1/200) (Enzo Life Science), anti-Phospho-Serine/Threonine (Upstate), anti-mCherry (1/500) (Clontech) and anti-RFP23 (link) (1/1,000) antibodies were used in this study.
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2

Mitochondrial Dynamics and Apoptosis Regulation

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Anti-Tim23 (1/1000), anti-Tom20 (for western blots, 1/1000, for immunofluorescence, 1/200), anti-Bcl2-L-13 (1/1000) (Proteintech), anti-cytochrome c (1/1000) (BD biosciences), anti-Bcl2-L-13 (1/500), anti-GAPDH (1/1000), anti-Fis1 (1/1000), anti-α-tubulin (1/1000) (Abcam), anti-LC3B (for western blots, 1/1000, for immunofluorescence, 1/100), anti-HA (for western blots, 1/1000, for immunofluorescence, 1/200), anti-Drp1Ser637 (1/500), anti-Drp1Ser616 (1/1000), anti-Mfn2 (1/1000), anti-cleaved caspase 3 (1/1000) (Cell Signalling Technology), anti-ATP synthase subunit beta (for western blots, 1/1000, for immunofluorescence, 1/200) (Life Technologies), anti-Drp1 (for western blots, 1/1000, for immunofluorescence, 1/100), anti-Opa1 (1/1000) (BD Transduction Laboratories), anti-FLAG (for immunofluorescence, 1/200) (Sigma), anti-Mfn1 (1/1000) (Abnova), anti-ubiquitin (for immunofluorescence, 1/200) (Enzo Life Science), anti-Phospho-Serine/Threonine (Upstate), anti-mCherry (1/500) (Clontech) and anti-RFP23 (link) (1/1000) antibodies were used in this study.
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3

Mitochondrial Dynamics in Acupuncture

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Instruments needed are disposable acupuncture needles (0.2x13mm, Huanqiu, Suzhou, China), and HANS acupoint nerve stimulator (LH202H, Huawei Industry Development, Beijing, China). ATP assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The following primary antibodies were used in this study: anti-TOM20, anti-TIM23, anti-Pink1, anti-Parkin (Proteintech, Chicago, IL, USA), anti-LC3 (MBL, Tokyo, Japan), anti-GAPDH (Abcam, Boston, Mass, USA), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Servicebio, Wuhan, China), horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Servicebio, Wuhan, China), Alexa Fluor 594 labeled goat anti-rabbit IgG (ZSGB-BIO, Beijing, China), Alexa Fluor 488 labeled goat anti-mouse IgG (ZSGB-BIO, Beijing, China), Prestained Protein Marker II (10–200kDa) (Servicebio, Wuhan, China).
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