Brdu b44

Manufactured by BD

Sourced in United States

BrdU (B44) is a laboratory reagent used for the detection of cellular proliferation. It is a synthetic nucleoside that can be incorporated into the newly synthesized DNA of replicating cells, acting as a marker for cell division.

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Lab products found in correlation

Ab39670, by Abcam (2 mentions) Icos c398.4a, by BioLegend (2 mentions) Foxp3 fjk 16s, by Thermo Fisher Scientific (2 mentions) Cd25 7d4, by BD (2 mentions) Il 17a tc11 18h10, by BD (2 mentions) Ifn g xmg1, by BD (2 mentions) Lag3 46 2231 80, by Thermo Fisher Scientific (2 mentions) Nrp1 fab566a, by R&D Systems (2 mentions) Ctla4 14d3, by Thermo Fisher Scientific (2 mentions) Gitr dta 1, by Thermo Fisher Scientific (2 mentions) P27 sc 776, by Santa Cruz Biotechnology (2 mentions) Ab85047, by Abcam (2 mentions) P foxo1 9461s, by Cell Signaling Technology (2 mentions) Ab5454, by Abcam (2 mentions) Foxo1, by Abcam (2 mentions) Cd62l mel 14, by BioLegend (2 mentions) Cd44 im7, by BioLegend (2 mentions) Raptor, by Abcam (2 mentions) Ki67 sola15, by Thermo Fisher Scientific (2 mentions) Cd4 h129, by BD (2 mentions)

Close spelling variants of this product

Anti-BrdU B44 (6 citations) Anti-BrdU (clone B44) (4 citations) Mouse anti-BrdU Clone B44 (3 citations) Anti-BrdU FITC (clone B44; (3 citations) FITC)-conjugated anti-BrdU antibody (B44; (2 citations)

5 protocols using brdu b44

Multiparameter FACS and Cell Sorting

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The following antibodies were used in fluorescence-activated cell sorting (FACS) and cell sorting: CD45.1 (A20, BioLegend), CD45.2 (104, BioLegend), CD4 (H129.19, BD Pharmingen), CD25 (7D4, BD Pharmingen), IL-17A (TC11-18H10.1, BD Pharmingen), IFN-g (XMG1.2, BD Pharmingen), CD44 (IM7, Bio-Legend), CD62L (MEL-14, BioLegend), CD25 (7D4, BD Pharmingen), Lag3 (46-2231-80, eBioscience), Nrp1 (FAB566A, R&D Systems), Ki67 (SolA15, eBioscience), CTLA4 (14D3, eBioscience), GITR (DTA-1, eBioscience), ICOS (C398.4A, BioLegend), Foxp3 (FJK-16 s, eBioscience), p27 (sc-776, Santa Cruz Biotechnology), p-p27 (ab85047, Abcam), Foxo1 (ab39670, Abcam), p-Foxo1 (9461S, Cell Signaling Technology), Raptor (ab5454, Abcam), Brdu (B44, BD Pharmingen).

Wu C., Chen Z., Xiao S., Thalhamer T., Madi A., Han T, & Kuchroo V. (2018). SGK1 Governs the Reciprocal Development of Th17 and Regulatory T Cells. Cell reports, 22(3), 653-665.

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Isolation and Characterization of Lymphocytes

Cited 2 times

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Lymphocytes were isolated from peripheral tissues as previously described (23 (link)). Isolated lymphocytes were stimulated with LCMV gp_33–41 peptide (1µg/ml) for 4hrs at 37°C and cytokine secretion was evaluated as described earlier (35 (link)). Single cell suspensions were stained with antibodies against CD69 (H1.2F3), CD44 (IM7), Thy1.1 (OX-7), CD62L (MEL-14) from Biolegend, CD8a (53–6.7), CD103 (M290), CD45.1 (A20), CD45.2 (104), IFNγ (XMG1.2), TNFα (MP6-XT22), IL2 (JES6-5H4) from eBioscience and BrdU (B44) from BDBiosciences. Samples were acquired on a Fortessa flow cytometer (BD Biosciences). Immunofluorescence was performed as described(36 (link)). Antibodies against Thy1.1 (OX-7), CD45.1 (A20), and CD8β (YTS156.7.7) from Biolegend were used to stain sections.
For in situ tetramer staining, mouse female reproductive tracts were manually cut into ~500 µm sections using a surgical blade. Tissue pieces were incubated with PE conjuagted tetramers in a 2% fetal bovine serum in phosphate buffered saline (PBS) overnight at 4°C. The next day, tissues were washed with ice cold PBS three times on ice. Tissue pieces were then fixed in 2% paraformaldehyde in PBS for 2 hours, then washed in PBS and then placed in a 30% sucrose PBS solution overnight. The next day tissue pieces were frozen in OCT.

Schenkel J.M., Fraser K.A., Casey K.A., Beura L.K., Pauken K.E., Vezys V, & Masopust D. (2016). IL-15 independent maintenance of tissue resident and boosted effector memory CD8 T cells. Journal of immunology (Baltimore, Md. : 1950), 196(9), 3920-3926.

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Multiparameter FACS and Cell Sorting

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Wu C., Chen Z., Xiao S., Thalhamer T., Madi A., Han T, & Kuchroo V. (2018). SGK1 Governs the Reciprocal Development of Th17 and Regulatory T Cells. Cell reports, 22(3), 653-665.

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Immunohistochemical Analysis of Cell Signaling Pathways

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Flag-Tag (F3165, 1:1000) and actin (A2066, 1:1000) were obtained from Sigma. BrdU (B44, 1:200) and Ki67 antibodies (556528, 1:300) were obtained from BD Pharmingen (San Jose, CA, USA). Caspase-2 (11B4, 1:500) was obtained from Alexis (Farmingdale, NY, USA). Mdm2 N-terminal (IF2, 1:200) and p53 (DO-1, 1:2000) were obtained from Calbiochem (Billerica, MA, USA). p21 (F-5, 1:100), p53 (FL393, 1:1000) and Mdm2 (SMP14, 1:1000) were obtained from Santa Cruz (Dallas, TX, USA). Cleaved Caspase-3 (9661, 1:500), phospho-Chk2 (T68) (2661, 1:1000), and Parp (9532, 1:1000) were obtained from Cell Signaling Technology (Danvers, MA, USA).

Terry M.R., Arya R., Mukhopadhyay A., Berrett K.C., Clair P.M., Witt B., Salama M.E., Bhutkar A, & Oliver T.G. (2014). Caspase-2 impacts lung tumorigenesis and chemotherapy response in vivo. Cell Death and Differentiation, 22(5), 719-730.

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Immunohistological Characterization of Tissue

Cited 1 time

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Immunohistology studies were carried out as described (Pawlisz et al., 2008 (link)) on 12 μm frozen or 5 μm paraffin sections. The following antibodies were used: FLNA, Pals 1, Igf2 (Epitomics); Cux1, DCX (Santa Cruz); Pax6, Neurofilament, ZO1, NaK ATPase (Developmental Study Hybridoma Bank); BrdU B44, βCatenin, E-Cadherin, N-Cadherin, VE-Cadherin, PECAM, Fibronectin, beta Integrin (BD Biosciences); Tuj1, Pericentrin (Covance); BrdU BU1/75, Foxp1, Foxp2, SATB2, Ctip2, Glast, (Abcam, Cambridge, MA); GFAP (Dako); NeuN, Tbr2, Tbr1, HABP (Millipore); panCadherin, GFP (Life Technologies); MAP2, Biotin-IB4 (Sigma); PDGFRβ (eBioscience); Alexa Fluor 488 Phalloidin and Alexa Fluor 546 Phalloidin and fluorescence conjugated secondary antibodies were from Life Technologies. All experiments were repeated with at least three independent litters and representative images are shown.

Houlihan S.L., Lanctot A.A., Guo Y, & Feng Y. (2016). Upregulation of neurovascular communication through filamin abrogation promotes ectopic periventricular neurogenesis. eLife, 5, e17823.

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