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Pcmv6 entry vector ps100001

Manufactured by OriGene
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Sourced in United States

The PCMV6-Entry vector (PS100001) is a cloning vector used for the expression of target genes in mammalian cells. It contains a cytomegalovirus (CMV) promoter to drive the expression of the inserted gene and a multiple cloning site for the insertion of the target DNA sequence.

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Lab products found in correlation

Sc 108080, by Santa Cruz Biotechnology (1 mentions) Amaxa nucleofector, by Lonza (1 mentions)

Spelling variants of this product

PCMV6-Entry vector (132 citations) PCMV6-Entry (97 citations) PS100001 (49 citations) PCMV6 vector (40 citations) PCMV6-Entry (PS100001; (8 citations)

2 protocols using pcmv6 entry vector ps100001

1

Inducible Expression of UEV1A Mutant

Cited 2 times
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The human UEV1A open reading frame (ORF) was amplified and cloned into a Dox-inducible Tet-ON plasmid pcDNA4.0/TO/HA(+) as described previously [20 (link)]. The mutated Ubc13-binding site (F38E) in Uev1A was designed based on a previous study with Mms2-F13E [7 (link)]. FOXO1 (MYC-DDK-tagged)-human forehead box O1 (NM_002015) plasmid (RC200477) and pCMV6-Entry vector (PS100001) were from Origene. The human BIM ORF was PCR-amplified as a KpnI-XhoI fragment and cloned into plasmid pcDNA4.0/TO/HA(+). The modified sequence for UEV1 shRNA (sc-38606-v) and negative control shRNA (sc-108080) delivered by lentiviral particles were from Santa Cruz Biotechnology, Inc. The lentiviral particle infection of colon cancer cells was performed following instructions of the supplier.
Wu Z., Niu T, & Xiao W. (2019). Uev1A promotes breast cancer cell survival and chemoresistance through the AKT-FOXO1-BIM pathway. Cancer Cell International, 19, 331.
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2

Transfection of UBE6T15 cells

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The pCMV6-Entry vector (PS100001) and HEY1 expression vector (RC200257) were purchased from OriGene Technologies (Rockville, MD, USA). All plasmids were transfected into UBE6T15 cells by using electroporation (Amaxa Nucleofector; Lonza, Tokyo, Japan).
Tsuru A., Setoguchi T., Matsunoshita Y., Nagao-Kitamoto H., Nagano S., Yokouchi M., Maeda S., Ishidou Y., Yamamoto T, & Komiya S. (2015). Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression. British Journal of Cancer, 112(7), 1232-1240.
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