6210 tof lc ms mass spectrometer

¹H and ¹³C spectra were recorded on Varian Unity VXR-300 (Varian Inc., Palo Alto, CA) and Bruker AV400 (Bruker, Karlsruhe, Germany) instruments. Chemical shifts (δ, ppm) were measured relative to residual ¹H and ¹³C resonances for CDCl₃ used as solvent. ESI-TOF analysis was carried out in an Agilent 6210 TOF LC/MS mass spectrometer (Agilent, Santa Clara, CA). UV-vis absorbance spectra were acquired using a Jasco V-650 spectrophotometer (Jasco, Cremella, Italy). Fluorescence emission spectra were obtained on an LS 55 fluorescence spectrometer (PerkinElmer, Waltham, MA, USA) at 25°C.

All the chemicals and solvents of analytical grade were purchased from commercial sources and were used as such without further purification. The reactions were performed under normal atmospheric conditions. The melting points were recorded on electrothermal 1101D Mel-Temp digital melting point apparatus. ¹H-NMR and ¹³C-NMR spectra were recorded on 400 MHz and 100 MHz Bruker spectrometer respectively in deuterated solvent containing TMS as internal standard. Chemical shift values are given in part per million (ppm). High-resolution mass spectra were obtained on an Agilent 6210 TOF LC/MS mass spectrometer.