Anti ogt sc 32921

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Anti-OGT (sc-32921) is a primary antibody produced by Santa Cruz Biotechnology. This antibody is reactive towards OGT, an enzyme responsible for the post-translational modification of proteins by the addition of O-linked N-acetylglucosamine (O-GlcNAc).

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Lab products found in correlation

Anti hcf 1 a301 400a, by Fortis Life Sciences (2 mentions) Anti nrf2 sc 13032, by Santa Cruz Biotechnology (1 mentions) Anti tubulin t9026, by Merck Group (1 mentions) Anti nrf1 d5b10, by Cell Signaling Technology (1 mentions) Anti o glcnac rl2 ab2739, by Abcam (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Calf alkaline phosphatase, by New England Biolabs (1 mentions) Udp glcnac u4375, by Merck Group (1 mentions) Anti o glcnac rl2 antibody, by Abcam (1 mentions) Lysozyme, by Roche (1 mentions) Anti rbbp5 a300 109a, by Fortis Life Sciences (1 mentions) Anti h3k4me1 13 0040, by EpiCypher (1 mentions)

Spelling variants of this product

Anti-OGT (6 citations) Sc-32921 (2 citations)

3 protocols using anti ogt sc 32921

Immunoblot Analysis of Protein Interactions

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Immunoblot analyses were performed as described previously (59 (link)). The antibodies that were used were anti-FLAG (F7425; Sigma), anti-OGT (sc-32921; Santa Cruz), anti-HCF-1 (A301-400A; Bethyl Lab), anti-NRF1 (D5B10; Cell Signaling), anti-O-GlcNAc/RL2 (ab2739; Abcam), anti-6×His (9C11; Wako), anti-GST (5A7; Wako), anti-β-TrCP (D13F10; Cell Signaling), anti-NRF2 (sc-13032; Santa Cruz), and antitubulin (T9026; Sigma).

Sekine H., Okazaki K., Kato K., Alam M.M., Shima H., Katsuoka F., Tsujita T., Suzuki N., Kobayashi A., Igarashi K., Yamamoto M, & Motohashi H. (2018). O-GlcNAcylation Signal Mediates Proteasome Inhibitor Resistance in Cancer Cells by Stabilizing NRF1. Molecular and Cellular Biology, 38(17), e00252-18.

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Synthesis and Characterization of UDP-GlcNAc Analogues

Cited 1 time

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UDP-GlcNAc (U4375) was obtained from Sigma, whereas thio-analogues of UDP-GlcNAc (UDP-5S-GlcNAc, S_p-αS-UDP-GlcNAc, and R_p-αS-UDP-GlcNAc) were synthesized as described (5 (link)). Calf alkaline phosphatase was obtained from New England Biolabs. Peptides (Table S1) were obtained from Biomatik. Anti-OGT (sc-32921) and anti-CK2α (sc-12738) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX), and anti-O-GlcNAc (RL2) antibody was purchased from Abcam (Cambridge, UK). Ni-NTA superflow and DTT were purchased from Sigma-Aldrich, and lysozyme was purchased from Roche Applied Science.

Kapuria V., Röhrig U.F., Waridel P., Lammers F., Borodkin V.S., van Aalten D.M., Zoete V, & Herr W. (2018). The conserved threonine-rich region of the HCF-1PRO repeat activates promiscuous OGT:UDP-GlcNAc glycosylation and proteolysis activities. The Journal of Biological Chemistry, 293(46), 17754-17768.

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Chromatin Remodeling Protein Analysis

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Anti-BRG1 (ab110641), anti-FOXK1 (ab18196), anti-OCT4 (ab19857) and anti-H3K27ac (ab4729) were from Abcam. Anti-T7 (D9E1X, 13246), Anti-H2AK119ub (D27C4), Anti H3 (1B1B2) and Anti-ASXL2 (E6Z3X) were from Cell Signaling Technology. Anti-ASXL2 (A302-037A), anti-HCF1 (A301-400A), and anti-RbBP5 (A300-109A) was from Bethyl Laboratories. Anti-ASXL1 (GTX127284) was from GeneTex. Anti-OGT (sc-32921) was from Santa Cruz. Anti-H3K4me1 (13-0040) was from EpiCypher. Anti-UTX, Anti-HA, Anti-Myc, and anti-MLL4 antibodies were homemade.

Zhang Y., Xie G., Lee J.E., Zandian M., Sudarshan D., Estavoyer B., Benz C., Viita T., Asgaritarghi G., Lachance C., Messmer C., Simonetti L., Sinha V.K., Lambert J.P., Chen Y.W., Wang S.P., Ivarsson Y., Affar E.B., Côté J., Ge K, & Kutateladze T.G. (2024). ASXLs binding to the PHD2/3 fingers of MLL4 provides a mechanism for the recruitment of BAP1 to active enhancers. Nature communications, 15(1).

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