Ovarian carcinoma SKOV3 cell lines were obtained from the Shanghai Institute of Cell Library (Shanghai, China). The small interfering RNA (siRNA) was designed and constructed as a vector (plasmid without vector and bacterial strain containing this plasmid were all produced and identified by Nanjing Moji Biological Technology Development Co. Ltd., Nanjing, China). The main reagents used were: CCK-8, IL-8, RPMI-1640 basal culture medium, double-antibody and fetal bovine serum (FBS) (both from Gibco, Grand Island, NY, USA), acrylamide, methylene acrylamide, Tris-base, ammonium peroxydisulfate, TEMED, glycine and SDS (all from Biosharp, Hefei, China). The PVDF membrane, and chemiluminescent liquid were purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA).
Sodium dodecyl sulfate (sds)
Manufactured by Biosharp
Sourced in China
SDS is a laboratory equipment used for the separation and analysis of proteins. It functions by disrupting protein structure and applying an electric charge, allowing for the separation of proteins based on their molecular weight.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Acrylamide, by Biosharp (2 mentions)
Glycine, by Biosharp (2 mentions)
Tris base, by Biosharp (2 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Double antibody, by Thermo Fisher Scientific (1 mentions)
Cck 8, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Reverse transcription kit, by Takara Bio (1 mentions)
Mda test kit, by Nanjing Jiancheng (1 mentions)
E z n a mollusc dna kit, by Omega Bio-Tek (1 mentions)
Taq pcr starmix, by GenStar (1 mentions)
Tb green premix ex taq 2 2, by Takara Bio (1 mentions)
Gelatin, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Sodium alginate, by Solarbio (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Collagenase type 1, by Merck Group (1 mentions)
Trizol reagent, by Takara Bio (1 mentions)
Bromophenol blue, by Thermo Fisher Scientific (1 mentions)
6 protocols using sodium dodecyl sulfate (sds)
1
SKOV3 Cell Line Knockdown Protocol
2
Comprehensive Biochemical and Molecular Analysis
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SDS was purchased from Biosharp Company of China, and the purity was 99%. Total protein quantitation kit, SOD, CAT, and MDA test kits were purchased from Nanjing Jiancheng Company of China for determination of the activities of SOD, CAT and MDA content. The E.Z.N.A.® Mollusc DNA kit was the product of Omega Bio-Tek Company for extraction of genomic DNA, and 2 × Taq PCR StarMix was purchased from GenStar Company. Trizol reagent was purchased from Thermo Fisher Technology Co., Ltd. for extraction of RNA. Reverse Transcription kit and TB Green premix Ex Taq II (2×) were purchased from TaKaRa Company. The sequences of 13 random primers and qPCR primers used in this study are shown in Tables S1 and S2 .
3
Liver Extracellular Matrix Extraction
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Liver tissues from C57 mice aged 6 to 8 weeks underwent isolation and perfusion with PBS. Subsequently, they were incubated in PBS containing 1% SDS (Biosharp) and 1% Triton X-100 (Diamond) for 72 hours for decellularization. The acellular liver tissue was freeze dried, and the resulting powder was dissolved in PBS containing 3% acetic acid and pepsin (1 mg/ml) (Sigma-Aldrich). This solution was then shaken at 37°C for 72 hours. NaOH was used to adjust the pH of the dissolved liver acellular matrix to neutral. After filtering out insoluble impurities using a mesh, the LDCM is stored at 4°C. Gelatin (Sigma-Aldrich) and sodium alginate (Solarbio) were dissolved in PBS, respectively, and stored at 4°C after sterilization. Before use, the liquid was heated to 37°C until it melted.
4
Two-dimensional Proteomic Analysis of Cell Lines
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Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from Gibco (Waltham, MA, USA), and fetal bovine serum (FBS) was also purchased from Gibco. Urea, CHAPS zwitterionic detergent (CHAPS), dithiothreitol (DTT), immobilized pH gradient (IPG) buffer, Tris-HCl, and IPG strips (17 cm, pH 4–7, linear) were purchased from Bio-Rad (Hercules, CA, USA). Collagenase (Type I), protease inhibitory cocktail, and thioUrea were purchased from Sigma-Aldrich (St Louis, MO, USA). Sodium dodecyl sulfate (SDS), glycerol, Tris base, agarose, glycine, and Coomassie R-250 were purchased from Biosharp (Hefei, China). Iodoacetamide was purchased from TCI (Portland, OR, USA). Bromophenol blue was purchased from Fisher Scientific (Pittsburgh, PA, USA). TRIzol reagent and reverse transcription kit were purchased from Takara (Shiga, Japan). Fast SYBR Green Master Mix was purchased from Toyobo (Osaka, Japan).
5
DNA Extraction from Buccal Swabs
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A total of 33 samples were purchased from Thailand, Indonesia and China by Guangzhou Tongkang Pharmaceutical Co., Ltd. (Table 1 ). Morphological identification was performed by Professor Xiaoping Lai and Professor Geng Li (Table 2 ). All samples were deposited in the Laboratory Animal Center of Guangzhou University of Chinese Medicine for future use. Cetyltrimethylammonium bromide (CTAB), Tris-base, Triton X-100, dichloromethane, isopropanol and Polyvinylpyrrolidone (PVP) were from Sigma-Aldrich Co., Ltd. (St. Louis, MO, United States). Sodium dodecyl sulfate (SDS) and ethylenediamine tetraacetic acid (EDTA) were from Biosharp Co., Ltd. (Hefei, Anhui, China). Buccal swab DNA extraction kit was from TIANGEN BIOTECH Co., Ltd. (Beijing, China). Guanidine isothiocyanate was from Solarbio Co., Ltd. (Beijing, China). Tris-HCl buffer and Tris-saturated phenol were from Leagene Biotechnology Co., Ltd. (Beijing, China). Dithiothreitol (DDT) was from Beyotime Co., Ltd. (Shanghai, China). Isoamylol and ethanol were from Sinopharm Chemical Reagent Co., Ltd. (Ningbo, China).
6
Crayfish Protein Analysis Protocol
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Fresh crayfish (P. clarkii) was purchased from the local market. Maleic acid (HPLC), ortho-phthalaldehyde (OPA, purity ≥ 98%) and DL-Serine (≥98%) from Macklin Co., Ltd. (Shanghai, China); N,N,N’,N’-Tetramethylethylenediamine (TEMED), Tris base (≥99.9%), β-mercaptoethanol (≥99%), and Coomassie brilliant blue R-250 from Sigma-Aldrich (St. Louis, MO, USA); acrylamide (≥99.5%), N,N-methylene bisacrylamide (≥99%), and Sodium dodecyl sulfate (SDS) from BioSharp Co., Ltd. (Shanghai, China); glycine and protein standards for SDS-PAGE from TAKARA Co., Ltd. (Tokyo, Japan); DL-dithiothreitol (DTT, purity ≥ 99%) and Coomassie brilliant blue G-250 from Fei Yang Biotechnology Co., Ltd. (Xi’an, China). All other reagents were of analytical grade.
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