Limonin from colonic digesta and mucosa was extracted based on the methods by Liang et al. 25 (link). The extracts were re-dissolved in 50% acetonitrile for LC-MS analysis (Model 2020, Shimadzu, Kyoto, Japan) with a negative ionization mode on a Zorbax SB-Aq C 18 column (150 mm × 4.6 mm, 5 µm, Agilent Technologies, USA) at a flow rate of 0.80 mL/min. The linear gradient elution condition was: 80% mobile phase A (5% ACN/water, v/v)/20% mobile phase B (100% ACN) (v/v) for 5 min initially, then shifted to 80% B/20% A over 30 min and held at 80% B for an additional 5 min. The elution was monitored on a selected m/z− of 469.
Model 2020
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu Model 2020 is a versatile laboratory instrument designed for analytical applications. It provides accurate and reliable measurements for a range of samples. The core function of the Model 2020 is to perform quantitative analyses, but the specific details of its intended use are not provided in this factual and unbiased description.
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Lab products found in correlation
3 protocols using model 2020
1
Limonin Extraction and LC-MS Analysis
2
Quantitative Metabolite Analysis in Mouse Gut
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Samples for LC-MS analysis were prepared as we described previously (19 (link), 20 (link)). Briefly, aliquots of the mucosa of mouse stomach, small intestine, cecum, and colon were homogenized with 50% methanol by Bead Ruptor Homogenizer (Omni International, Kennesaw GA). All sulfated and glucuronide metabolites were measured by enzymatic hydrolysis of the processed samples with β-glucuronidase and sulfatase as described (20 (link), 21 (link)). Then the homogenates were extracted with equal volume of ethyl acetate for 3 times. The ethyl acetate extracts were combined and dried using a vacuum concentrator (Model: SVC 100H, Thermo Fisher Scientific Inc.), and then resuspend in 50% methanol for LC-MS (Model 2020, Shimadzu, Kyoto, Japan) analysis. Quantitation by LC-MS was performed by SIM, using ESI mode as described previously (22 (link)).
3
Quantification of Colonic ATST, NBT and Metabolites
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