Anti c ebpβ antibody sc 150

Anti-apoB antibody (ab7616) was obtained from Abcam, anti-β-actin antibody (A5441) was obtained from Sigma, anti-C/EBPβ antibody (sc-150) was obtained from Santa Cruz Biotechnology, and anti-lamin antibody (2032) was obtained from cell signaling technology.

ChIP assay was operated under the manufacturer’s protocol (EZ-ChIP kit, 17-371RF, Millipore). In brief, primary microglia, adult microglia, or BV2 cells were fixed with 1% formaldehyde (F1635-500ML, Sigma-Aldrich) on the rotator for 10 minutes at room temperature and then quenched with 125 mM glycine on ice for another 10 minutes. After washing with PBS twice, cells were lysed with SDS lysis buffer, then cell lysates were sonicated with Covaris E220 for 10 minutes and precleared with Protein A agarose (16–157, Millipore). The sonicated and precleared cell lysates were immunoprecipitated with anti-C/EBPβ antibody (sc-150, Santa Cruz) or isotype IgG as control. After washing with buffers, chromatin was eluted from the Protein A/antibody/protein/DNA complex and reversed crosslinks with 5 M NaCl for 4 hours at 65 ºC. The freed DNA was digested with proteinase K and RNase A and purified with Phenol-Chloroform-Isoamyl Alcohol Mixture (Sangon Biotech). The DNA was then subjected to qPCR with SYBR Green master mix (04913914001, Roche). Sequences of ChIP-qPCR primers on mouse Cd36 promoter were listed on S1 Table.