Blood samples from fathers were collected using 4.5 ml tubes with EDTA (BD Vacutainer®Blood Collection System). Blood samples were put in the freezer (-20°C) immediately after collection. At delivery, umbilical cord blood was collected via umbilical vein puncture into 4.5 mL tubes containing EDTA (BD Vacutainer®Blood Collection System), followed by storage at -20°C. DNA extraction from whole blood samples was done using the Salting out method [32] . The quantity and purity of DNA was determined by a Nano Drop spectrophotometer. Extracted DNA was further stored in TE-buffer at -80°C until further analysis.
Vacutainer blood collection system
Manufactured by BD
Sourced in United Kingdom
The BD Vacutainer Blood Collection System is a medical device used for the collection and transportation of blood samples. It consists of a sterile evacuated tube, a holder, and a needle. The system is designed to facilitate the safe and accurate collection of blood specimens for laboratory testing.
Automatically generated - may contain errors
Lab products found in correlation
13 protocols using vacutainer blood collection system
1
Collection and Storage of Blood Samples
2
Blood Collection for Virus Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To collect blood samples, animals were sedated with Alfaxan (12 mg/kg, IM route). Blood was drawn from the femoral vein in the groin using aseptic techniques using a Vacutainer blood collection system (Becton Dickinson, Vacutainer systems). EDTA tubes with collected blood were centrifuged for 10 min at 1000 × g followed by the collection of plasma. A volume of 125 µl plasma was used for clinical chemistry measurements. The remaining material was stored at −80 °C for virus detection via PCR and for the detection of RVFV antibodies. Upon euthanasia, 2 ml EDTA blood and plain blood samples were collected. Serum samples were prepared by centrifugation of clotted blood for 10 min (1000–1300 × g), and serum was stored at −20 °C.
3
Standardized Blood Collection and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected by jugular venipuncture, using the Vacutainer blood collection system (Becton, Dickinson and Co., Rutherford, NJ). Serum was separated by centrifugation at 1000 × g at 4 °C for 15 min, within 1 h of collection, and aliquots were stored at − 80 °C until analysis.
The haematological parameters in whole blood (HGB, MCH, MCHC, MCV, HCT, RBC, RDW, WBC, MON, GRA, LYM, PLT, and MPV were analysed on the Abbott Cell-Dyn CD 3500 automated haematology analyser (Abbott Diagnostic division, Mountain View, CA).
Serum triglycerides, total cholesterol, and creatinine levels, as well as the activities of ALT, AST, ALP, CK, and LDH, were determined by standard commercial reagent packages (Beckman Coulter Biomedical Ltd., O’Callaghans Mills, Ireland) with the Beckman Coulter AU 680 biochemical analyser (Beckman Coulter Biomedical Ltd. München, Germany).
The haematological parameters in whole blood (HGB, MCH, MCHC, MCV, HCT, RBC, RDW, WBC, MON, GRA, LYM, PLT, and MPV were analysed on the Abbott Cell-Dyn CD 3500 automated haematology analyser (Abbott Diagnostic division, Mountain View, CA).
Serum triglycerides, total cholesterol, and creatinine levels, as well as the activities of ALT, AST, ALP, CK, and LDH, were determined by standard commercial reagent packages (Beckman Coulter Biomedical Ltd., O’Callaghans Mills, Ireland) with the Beckman Coulter AU 680 biochemical analyser (Beckman Coulter Biomedical Ltd. München, Germany).
4
Fasting Blood Lipid Panel Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following at least 8 h of overnight fasting, venous blood sample (~5 mL) was obtained using BD vacutainer blood collection system. The blood was kept in the refrigerator at 4 °C and transported to an accredited commercial biochemistry laboratory (Quest Laboratories, Singapore) within 2 h of the blood draw. Analyses for fasting glucose, fasting insulin, plasma triglyceride, low-density lipoprotein (LDL)-cholesterol and high-density lipoprotein (HDL)-cholesterol were performed using standardized procedures. The intra-assay coefficients of variation of these markers are within 3% (Quest Laboratories, Singapore).
5
Fasted Blood Lipid Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On a separate day, with each participant fasted for at least 8 hours the night before, approximately 5 mL of blood were collected using the BD Vacutainer blood collection system in the hospital and sent directly to the hospital biochemical laboratory. Triglyceride, low density lipoprotein (LDL)-cholesterol and high density lipoprotein (HDL)-cholesterol analyses were performed on a modular analyser (Roche Cobas 8000, Switzerland). The intra-assay coefficient of variation (CV) for blood lipids were all within 5%.
6
Plasma Preparation from Sodium Citrate Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood was collected in tubes containing 0.129 M sodium citrate (BD Vacutainer Blood Collection System) using 21-gauge needles (BD Vacutainer needles). Platelet-poor plasma was obtained within 60 min of sampling by centrifugation at 2,000 × g for 15 min at room temperature, then divided into aliquots and stored frozen at –70°C until further analysis.
7
Blood Sampling for Rheumatic Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood sampling was obtained in the same manner for all patients at the Internal Clinic, Department of Rheumatology, Clinical Center Kragujevac, Serbia. Venous blood from all participants was collected between 8 and 10 am, following at least a 10-hour fasting period, in a quiet, air-conditioned, and temperature-controlled room (22–24°C). Blood was collected in Vacutainer tubes containing 0.129 M sodium citrate (BD Vacutainer Blood Collection System) using 21-gauge polyethylene catheter for taking blood samples (BD Vacutainer needles). Blood was centrifuged to separate plasma and red blood cells (RBCs) and stored at −20°C.
8
RNA Isolation from Buffy Coat
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA isolation from the buffy coat obtained after centrifugation (150 g, 4 °C, 10 min.) was performed by the use of a commercial QIAamp® RNA Blood Mini set. Genetic material was isolated from 3.5 mL of peripheral blood obtained from all volunteers using EDTA tubes and the BD Vacutainer® Blood Collection system. The isolation process was fully compliant with the manufacturer’s guidelines. The isolation process was extended by an additional purification step using the RNase-Free DNase Set to obtain the purest product free of any genomic DNA. All of the procedures were carried out within no more than 2 h from the collection of a blood sample. Part of extracted RNA was used to visualize a product and obtain cDNA immediately after the isolation process; the rest of the genetic material was stored at −80 °C until analyzed.
9
Sodium Citrate Blood Plasma Collection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following at least a 10-h fasting period, blood was collected in Vacutainer tubes containing 0.129 M sodium citrate (BD Vacutainer Blood Collection System) using 21-gauge needles (BD Vacutainer needles). Platelet-poor plasma was obtained within 60 min of sampling by centrifugation at 2000×g for 15 min at room temperature and stored at -70 °C. The frozen plasma samples were transported to the Karolinska Institutet, Clinical Chemistry-Coagulation, Department of Molecular Medicine and Surgery, Stockholm, Sweden, for further analysis.
10
PBMC Isolation and Cryopreservation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected into lithium heparin-treated vacutainer blood collection systems (Becton Dickinson, UK). PBMC were isolated and used within 6 hours in fresh assays as previously described.65 (link) Excess cells were frozen in fetal calf serum (FCS) containing 10% dimethyl sulfoxide (DMSO) and stored in liquid nitrogen. Plasma samples were stored at −80°C. For serum preparation, untreated blood samples were stored at room temperature (RT) and then the clotted blood was centrifuged for 5 min (1000 xg). Serum was stored at −80°C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!