Cells were washed three times with ice-cold PBS and fixed with 4% paraformaldehyde-PBS. Cell were then incubated with 0.1% Triton-PBS for 15 minutes, 1% bovine serum albumin-PBS for 10 minutes, and then treated with goat anti-human Ki67 monoclonal antibody (1:1000, Santa Cruze, Texas, USA at 4°C overnight. FITC labeled mouse anti-goat IgG (1:1000, Santa Cruz, Texas, USA) was used for visualization.
Goat anti human ki67 monoclonal antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Goat anti-human Ki67 monoclonal antibody is a laboratory reagent used for the detection of the Ki67 protein, a cellular marker for proliferation. This antibody is produced in goats and is specific for the human Ki67 antigen.
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Lab products found in correlation
2 protocols using goat anti human ki67 monoclonal antibody
1
Quantifying Cellular Proliferation
2
Immunohistochemical Staining for Nrg1 and Ki67
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Endogenous peroxidase was neutralized with 3% H2O2 in methanol (10min) after antigen retrieval in a 0.1M critrate buffer (PH5.8) at 95°C for 5min and cooled at 25°C for 1h. Sections were blocked with normal human serum albumin (10min), then treated with the following primary antibodies overnight at 4°C: Rabbit anti-human Nrg1 polyclonal antibody(1:1000, Santa cruz, Texas); Goat anti human Ki67 monoclonal antibody ((1:1000, Santa cruz, Texas). After treatment with biotinylated secondary antibody, color reactions were performed with diaminobenzidine (DAB) (Sigma) and counterstained with Mayer’s hematoxylin.
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