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2 protocols using n octadecyltrichlorosilane

1

Synthesis and Characterization of Gold Nanoparticles

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Gold(III) chloride trihydrate, trisodium citrate dihydrate, poly-(allylamine hydrochloride) (PAH, MW ~56 kDa), n-octadecyltrichlorosilane (OTS, 90+%), α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), trifluoroacetic acid (TFA), titanium(IV) chloride, [Sar1, Thr8]-angiotensin II, and neurotensin were from Sigma-Aldrich (St. Louis, MO). Citric acid, sodium silicate (37%), ethanol, toluene, and chloroform were from Fisher Scientific (Pittsburgh, PA). Acetyl-tetradecapeptide renin substrate was from AnaSpec (San Jose, CA). BK-7 glass microscope slides were from Corning (Painted Post, NY).
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2

Reconstitution of Cholera Toxin Receptors

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Cholera toxin (CT) from Vibrio cholera, Triton X-100, 1-octadecanethiol (98%), and n-octadecyltrichlorosilane (OTS, 90+%) were from Sigma-Aldrich (St. Louis, MO). Trehalose was from Swanson Health Products (Fargo, ND).
1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-|6-[(7-nitro-2−1,3-benzoxadiazol-4-yl)amino]hexanoyl}-sn-glycero-3-phosphocholine (NBD-PC) were from Avanti Polar Lipids (Alabaster, AL). Monosialoganglioside GM1 (NH4+ salt) and monosialoganglioside GM2 (NH4+ salt) were from Matreya (Pleasant Gap, PA). Monosialoganglioside GM3 was from EMD Biosciences (La Jolla, CA). BK-7 glass substrates were from Corning (Painted Post, NY). Chromium and gold used for electron-beam evaporation were acquired as pellets of 99.99% purity from Kurt J. Lesker (Jefferson Hills, PA).
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