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Aav dj ef1adiogcamp6m

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The AAV-DJ-EF1aDIOGCaMP6m is a viral vector designed for the expression of the GCaMP6m calcium indicator in cells. It utilizes the EF1α promoter to drive the expression of the DIO (double-floxed inverted open reading frame) GCaMP6m construct packaged within an adeno-associated virus serotype DJ (AAV-DJ) backbone.

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Lab products found in correlation

Aav5 hsyn dio hm3d gq mcherry, by Addgene (2 mentions) Aav5 ef1a dio hchr2 h134r eyfp, by Addgene (2 mentions) Aav5 ef1a dio eyfp, by Addgene (2 mentions) Aav5 hsyn dio hm4d gi mcherry, by Addgene (1 mentions) Aav5 hsyn dio mcherry, by Addgene (1 mentions)

2 protocols using aav dj ef1adiogcamp6m

1

Viral Vector Toolbox Protocols

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The following AAV vectors (with a titer of >1012) were purchased from the vector core at the University of North Carolina at Chapel Hill (UNC Vector Core): AAV5-EF1a-DIO-hChR2(H134R)-EYFP, AAV5-EF1a-DIO-EYFP, and AAV-DJ-EF1aDIOGCaMP6m. The following AAV vectors were from Addgene: AAV5-hSyn-DIO-hM3D(Gq)-mCherry (no. 44361), AAV5-hSyn-DIO-hM4D(Gi)-mCherry (no. 44362), and AAV5-hSyn-DIO-mCherry (no. 50459).
Zhao Z.D., Han X., Chen R., Liu Y., Bhattacherjee A., Chen W, & Zhang Y. (2022). A molecularly defined D1 medium spiny neuron subtype negatively regulates cocaine addiction. Science Advances, 8(31), eabn3552.
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2

Diverse Viral Vectors for Optogenetics

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The following AAV vectors (with a titer of >1012) were purchased from UNC Vector Core: AAV5-EF1a-DIO-hChR2(H134R)-EYFP, AAV5-EF1a-DIO-EYFP, AAV-DJ-EF1a-DIO-GCaMP6m. The following AAV vectors were purchased from Addgene: AAV5-hSyn-DIO-hM3D(Gq)-mCherry (#44361), AAV5-hSyn-DIO-hM4D(Gi)-mCherry (#44362), AAV5-hSyn-DIO-mCherry (#50459), pAAV-flex-taCasp3-TEVp (#45580), pAAV-Ef1α-DIO eNpHR 3.0-EYFP (#26966), EnvA G-deleted Rabies-EGFP (Salk Institute).
Liu Y., Zhao Z.D., Xie G., Chen R, & Zhang Y. (2023). A molecularly distinct accumbal-to-lateral hypothalamic circuit modulates food seeking and consumption. bioRxiv.
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