The mice were sacrificed by decapitation and the brains were quickly removed. The left halves of the brains were fixed in 4% paraformaldehyde and embedded in paraffin, while the right halves were quickly frozen and stored at −80°C for biochemical analysis. Immunohistochemistry was performed according to methods described previously.[19 (link)] For 6E10 staining, which detects Aβ, the blocking step was preceded by treatment with 70% formic acid for 10 min. The following primary antibodies were used: 6E10 (monoclonal mouse anti-Aβ1-16 1:1000, Biolegend, USA), polyclonal rabbit anti-Rab5 (1:300, Abcam, USA), polyclonal goat anti-Rab7 (1:100, Santa Cruz, USA), polyclonal rabbit anti-LC3B (1:200, Cell Signaling Technology, USA), polyclonal rabbit anti-Lamp1 (1:400, Abcam), polyclonal goat anti-Beclin1 (1:100, Santa Cruz), polyclonal rabbit anti-UVRAG (1:400, Millipore, USA), polyclonal rabbit anti-Rubicon (1:50, LifeSpan Biosciences, USA), Alexa Fluor-conjugated antibodies (Molecular Probes, USA), and HRP-conjugated antibodies (chromogenic). Nuclei were labeled with DAPI or hematoxylin. Images were acquired using a confocal microscope (Fluoview10, Olympus, Tokyo, Japan) or an epifluorescence microscope (Olympus).
Polyclonal goat anti beclin1
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Polyclonal goat anti-Beclin1 is a laboratory reagent used to detect the Beclin1 protein by immunological methods. Beclin1 is a key regulator of autophagy, a cellular process involved in the degradation and recycling of cellular components. This product can be used to study the expression and localization of Beclin1 in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Epifluorescence microscope, by Olympus (1 mentions)
Alexa fluor conjugated antibody, by Thermo Fisher Scientific (1 mentions)
Polyclonal rabbit anti rab5, by Abcam (1 mentions)
Rabbit polyclonal anti lc3b, by Cell Signaling Technology (1 mentions)
Polyclonal rabbit anti lamp1, by Abcam (1 mentions)
Polyclonal rabbit anti uvrag, by Merck Group (1 mentions)
Fluoview10, by Olympus (1 mentions)
Fatty acid kit, by Cayman Chemical (1 mentions)
Mouse insulin elisa kit, by Merck Group (1 mentions)
Mouse leptin elisa kit, by Merck Group (1 mentions)
Mouse monoclonal anti β actin, by Merck Group (1 mentions)
2 protocols using polyclonal goat anti beclin1
1
Immunohistochemical Analysis of Alzheimer's Pathology
2
Antibody-based Analysis of Cellular Pathways
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The following antibodies were used in this study: polyclonal goat anti-Bif-1 (GTX 21343, GeneTex); poly-clonal guinea pig anti-P62 (03-GP62-C, American Research Products); monoclonal rabbit anti-Plin1 (9349, Cell Signaling); polyclonal rabbit anti-Atg9a (GTX128427, Genetex); monoclonal rat anti-Lamp1 (19992, Santa Cruz); polyclonal rabbit anti-phospho-mTOR (S2448) (2971, Cell Signaling); monoclonal rabbit anti-mTOR (2983, Cell Signaling); monoclonal rabbit anti-phospho-Akt (S473) (9271, Cell Signaling); monoclonal mouse anti-Akt (MAB2055, R&D Systems); monoclonal rabbit anti-PPARγ (2443, Cell Signaling); monoclonal rabbit anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370, Cell Signaling); monoclonal rabbit anti-p44/42 MAPK (Erk1/2) (9102, Cell Signaling); polyclonal rabbit anti-LC3B (NB100-2220, Novus Biologicals); monoclonal mouse anti-Atg5 (M153-3, MBL); polyclonal goat anti-Beclin1 (sc-10086, Santa Cruz); monoclonal mouse anti-Ubiquitin (NP300-130, Novus Biologicals); monoclonal mouse anti-β actin (A5441, Sigma). Mouse leptin ELISA kit and mouse insulin ELISA kit were purchased from Millipore. Mouse diabetes Luminex multiplex assay kit was purchased from Bio-Rad. Fatty acid kit was purchased from Cayman Chemicals. Unless otherwise stated, all other chemicals were purchased from Sigma.
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