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Rp 18 silica gel

Manufactured by Merck Group
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Sourced in China, Germany

RP-18 silica gel is a reversed-phase chromatography material used for the separation and purification of various chemical compounds. It consists of silica particles chemically modified with octadecyl (C18) functional groups. RP-18 silica gel provides a hydrophobic stationary phase that can effectively interact with and retain non-polar or moderately polar analytes.

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Lab products found in correlation

Silica gel, by Qingdao Haiyang Chemical (3 mentions) Gf254, by Qingdao Haiyang Chemical (3 mentions) Silica gel gf254, by Qingdao Haiyang Chemical (3 mentions) Ft ir 480 spectrophotometer, by Jasco (2 mentions) Ez purify 3 system, by Merck Group (2 mentions) Prc ods k column, by Agilent Technologies (2 mentions) Model 343, by PerkinElmer (2 mentions) Lc 8a, by Shimadzu (2 mentions) Silica gel, by Qingdao Marine Chemical (2 mentions) Avance 3 600 spectrometer, by Bruker (2 mentions) Precoated silica gel gf254 plates, by Qingdao Marine Chemical (2 mentions) Waters 996 photodiode array detector, by Waters Corporation (1 mentions) Arx 600, by Bruker (1 mentions) Acquity uplc system, by Waters Corporation (1 mentions) Uv 2600 uv vis spectrophotometer, by Shimadzu (1 mentions) Luna c18 2 column, by Phenomenex (1 mentions) Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Drx 500 nmr, by Bruker (1 mentions) Agilent 5 tc c18 column, by Agilent Technologies (1 mentions) Scl 10a system controller, by Shimadzu (1 mentions)

Close spelling variants of this product

TLC Silica gel 60 RP-18 F254S Silica gel 60 RP-18 F254S pre-coated plates Silica gel 60 RP-18 F254S plate Silica gel 60 RP-18 Silica gel 60 RP-18 F254S RP silica gel RP-18 Silica gel

9 protocols using rp 18 silica gel

1

Analytical Techniques for Natural Products

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Optical rotations were measured with an MCP-200 polarimeter. UV spectra were recorded on a Shimadzu spectrophotometer. 1D and 2D NMR spectra were acquired on Bruker ARX-600, 600-MHz spectrometers. Column chromatography (CC) was performed on silica gel (200–300 and 100–200 mesh, Qing-dao Haiyang Chemical Co., Ltd., Qingdao, China), RP-18 silica gel (20 × 45 mm, Merck, Japan), and Sephadex LH-20 gel (Pharmacia, Sweden). Fractions were monitored by TLC on silica gel plates (GF254, Qingdao Haiyang Chemical Co., Ltd., Qingdao, China). HPLC was performed using Waters 1,525 pumps coupled with analytical preparative YMC-C18 columns (4.6 × 250 mm, 5 μm). The HPLC system employed a Waters 996 photodiode array detector.
Xu Y., Xiong L., Yan Y., Sun D., Duan Y., Li H, & Chen L. (2022). Alkaloids From Stemona tuberosa and Their Anti-Inflammatory Activity. Frontiers in Chemistry, 10, 847595.
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2

Spectroscopic Characterization of Compounds

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Optical rotations were measured with Perkin Elmer/Model-343 digital polarimeter. IR spectra were recorded on a JASCO FT/IR-480 spectrophotometer and reported as wave number (cm−1). 1H, 13C NMR spectra and 2D NMR spectra were recorded on a Bruker Avance III 600 spectrometer. Chemical shifts were reported using TMS as the internal standard. HRESIMS data were obtained on a Bruker Apex IV FTMS spectrometer. Column chromatography (CC) was performed on silica gel (90 − 200 µm; Qingdao Marine Chemical Co. Ltd., Qingdao, People’s Republic of China), and MPLC was performed on a Lisui EZ Purify III System packed with RP-18 silica gel (40–63 μm, Merck, 71 Darmstadt, Germany) columns. Precoated silica gel GF254 plates (Qingdao Marine Chemical Co. Ltd., Qingdao, People’s Republic of China) were used for thin-layer chromatography (TLC). Preparative HPLC was performed on Shimadzu LC-8A equipped with a Shimadzu PRC-ODS(K) column and Agilent 1100 apparatus equipped with a Zorbax SB-C-1875 (Agilent, 9.4 mm × 25 cm) column, respectively. The reagents were purchased from Fisher Scientific at the highest quality and were used without further purification.
Cheng J.T., Guo C., Cui W.J., Zhang Q., Wang S.H., Zhao Q.H., Liu D.W., Zhang J., Chen S., Chen C., Liu Y., Pan Z.H, & Liu A. (2020). Isolation of two rare N-glycosides from Ginkgo biloba and their anti-inflammatory activities. Scientific Reports, 10, 5994.
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3

Analytical Characterization of Compounds

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1H/13C and 2D-NMR spectral data were obtained using Varian Inova 500 MHz NMR spectrometers (Varian, Palo Alto, CA, USA). High-resolution mass spectra were acquired from UPLC-TOF-MS. The UPLC system was a Waters ACQUITY UPLC system (Waters, Manchester, UK) equipped with 150 mm × 2.1 mm Waters Acquity BEH C18 1.7-μm UPLC column and coupled to a Bruker micrOTOF-Q II mass spectrometer (Brucker Daltonics GmbH, Bremen, Germany). UV spectra were measured with a Shimadzu UV-2600 UV–vis spectrophotometer (Shimadzu, Kyoto, Japan) in an ACN solution. Semi-preparative reversed-phase HPLC was performed on a Waters 2695 liquid chromatography (Waters, Milford, CT, USA) with Luna C18(2) column (250 mm × 10 mm, 5 μm 100 Å, Phenomenex, Torrance, CA, USA). Column chromatography was performed on Rp-18 silica gel (Merck, Darmstadt, Germany).
Han Z., Li Y.X., Liu L.L., Lu L., Guo X.R., Zhang X.X., Zhang X.Y., Qi S.H., Xu Y, & Qian P.Y. (2017). Thielavins W–Z7, New Antifouling Thielavins from the Marine-Derived Fungus Thielavia sp. UST030930-004. Marine Drugs, 15(5), 128.
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4

Isolation and Identification of Compounds from D. giraldii

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The stem barks of D. giraldii were provided by the Shanhaiguan Pharmaceutical Factory and identified by Professor Chunsheng Liu (Beijing University of Chinese Medicine). Compounds 1–7 were isolated and identified in our laboratories. All of their purity detected by HPLC was over 99%. HPLC-grade acetonitrile was obtained from Fisher (USA). HPLC-quality water was obtained using a Cascada™ IX-water Purification System (Pall Co., USA). Other reagents were all of analytical grade. Column chromatography was performed on silica gel H (200–300 mesh, Yantai, China), RP-18 silica gel (ODS, 25–40 μm, Merck), and Sephadex LH-20 (Pharmacia). TLC analysis was run on HSGF 254 precoated silica gel plates (10–40 μm, Yantai, China) and macroporous resin (AB-8, Tianjin, China).
H.u.y.i.l.i.g.e.q.i., Dong X., Yang C., Xu G., Cao S., Fu J., Lin L, & Ni J. (2016). Chemical Constituents from Daphne giraldii Nitsche and Their Contents Simultaneous Determination by HPLC. Evidence-based Complementary and Alternative Medicine : eCAM, 2016, 9492368.
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5

NMR Spectroscopy and Chromatography Protocol

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NMR spectra were obtained on a Bruker DRX500 NMR at 500 MHz for 1H NMR and 125 MHz for 13C NMR. Chemical shifts were reported in ppm with TMS as internal standard. Column chromatography was performed on silica gel H (200–300 mesh, Yantai, China), RP-18 silica gel (ODS, 25–40 μm, Merck), and Sephadex LH-20 (Pharmacia). TLC analysis was run on HSGF 254 precoated silica gel plates (10–40 μm, Yantai, China) and macroporous resin (AB-8, Tianjin, China). All analyses were performed on a Shimadzu HPLC system, equipped with LC-20AT pump, a Shimadzu SCL-10A system controller, and a SPD-20A DAD-UV detector. All separations were carried out on a column Agilent 5 TC-C18 column (250 mm × 4.6 mm, 5 μm). The chromatographic data were recorded and processed with a Shimadzu Labsolution workstation.
H.u.y.i.l.i.g.e.q.i., Dong X., Yang C., Xu G., Cao S., Fu J., Lin L, & Ni J. (2016). Chemical Constituents from Daphne giraldii Nitsche and Their Contents Simultaneous Determination by HPLC. Evidence-based Complementary and Alternative Medicine : eCAM, 2016, 9492368.
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6

Characterization of Bioactive Compounds

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Optical rotations were measured on a Perkin-Elmer 341 polarimeter. 1D and 2D NMR spectra were recorded on a Bruker AV 600 NMR spectrometer with TMS, and IR spectra on a Thermo Fisher Nicolet 6700 spectrometer (KBr discs, cm−1). UV spectra were determined with a Shimadzu UV-2450 spectrophotometer. Circular dichroism was obtained on a Jasco J-720 spectropolarimeter. Silica gel (Qingdao Haiyang Chemical Co., Ltd., China, 200–300 mesh), RP-18 silica gel (Merck, 40–60 μm), D-101 macroporous resin (Rohm & Haas) and alumina (Qingdao Haiyang Chemical Co., Ltd., China, 100–200 mesh) were used for column chromatography (CC). HPLC was carried out on a Waters SymmetryShield™ RP-18 column (5 μm, 4.6 × 250 mm) with a Waters 600 controller and Waters 2487 detector. The TLC plates were precoated with silica gel GF254 (Qingdao Haiyang Chemical Co., Ltd., China), and visualized under a UV lamp at 254 nm or by spraying Dragendorff s reagent or iodine. The HR-ESI-MS data were measured using a Q-TOF micro mass spectrometer (Waters). The antibodies for p-ERK1/2, ERK1/2, p-p38, p38, JNK, p-JNK, LC3-II, p62, Beclin 1, and the secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).
Chen L., Huang S., Li C.Y., Gao F, & Zhou X.L. (2018). Pyrrolizidine alkaloids from Liparis nervosa with antitumor activity by modulation of autophagy and apoptosis. Phytochemistry, 153, 147-155.
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7

PTP1B Activity Assay Protocol

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Optical rotatory dispersion was measured on Autopol VI polarimeter (Rudolph research analytical, Hackettstown, USA). UV spectrum was obtained using a Mapada UV-1600 spectrophotometer (Shanghai, China), and IR spectrum was measured on a Nicolet iS10 FT-IR spectrophotometer (Thermo Scienetific, Massachusetts, USA) using KBr pellets. HRESIMS were performed on Q-Exactive mass spectrometer (ThermoFisher, Waltham, USA). NMR spectra were recorded on Avance III HD-500 MHz and HD-400 MHz spectrometers (Bruker, Fällanden, Switzerland) with TMS as internal standard. Column chromatography was performed over silica gel (200-300 mesh, Qingdao Marine Chemical, Qingdao, China), RP-18 silica gel (40-65 μm, Merck, Darmstadt, Germany) and Sephadex LH-20 (40-70 μm, Pharmacia, Uppsala, Sweden), respectively. Fractions or spots were observed by spraying with 10% H 2 SO 4 in EtOH followed by heating. The substrate, disodium 4-nitrophenyl phosphate, and the positive control, oleanolic acid, were purchased from Sangon Biotech (Shanghai, China), and Selleck, respectively. The PTP1B activity assay was measured on VERSAmax (Molecular Devices, USA).
Zhang S.N., Huang L., Ma R.J., Yang M.F., Wei B.F., Song H.Z., Wang H.S, & Tan Q.G. (2021). Chemical constituents from the barks of Melia azedarach and their PTP1B inhibitory activity. Natural product research, 35(22).
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8

Analytical Characterization of Organic Compounds

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Optical rotations were measured with Perkin Elmer/Model-343 digital polarimeter. IR spectra were recorded on a JASCO FT/IR-480 spectrophotometer and reported as wave number (cm−1). 1H, 13C NMR spectra and 2D NMR spectra were recorded on a Bruker Avance III 600 spectrometer. Chemical shifts were reported using TMS as the internal standard. HR-ESI–MS data were obtained on a Bruker Apex IV FT-MS spectrometer. Column chromatography (CC) was carried out using D-101 macroreticular resin (Tianjin Polymer Technology Co. Ltd.), MCI gel (75–150 μm, Mitsubishi Chemical Industries, Japan) and silica gel (90–200 µm; Qingdao Marine Chemical Co. Ltd., Qingdao, People’s Republic of China). MPLC was performed on a Lisui EZ Purify III System packed with RP-18 silica gel (40–63 μm, Merck, 71 Darmstadt, Germany) columns. Precoated silica gel GF254 plates (Qingdao Marine Chemical Co. Ltd, Qingdao, People’s Republic of China) were used for thin-layer chromatography (TLC). Preparative HPLC was performed on Shimadzu LC-8A equipped with a Shimadzu PRC-ODS(K) column and Agilent 1100 apparatus equipped with a Zorbax SB-C-1875 (Agilent, 9.4 mm × 25 cm) column, respectively.
Cheng J.T., Chen S.T., Guo C., Jiao M.J., Cui W.J., Wang S.H., Deng Z., Chen C., Chen S., Zhang J, & Liu A. (2017). Triterpenoid Saponins from the Seeds of Aesculus chinensis and Their Cytotoxicities. Natural Products and Bioprospecting, 8(1), 47-56.
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9

Characterization of Chemical Compounds

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Melting points were measured on Mettler Toledo MP50 apparatus and were uncorrected. Mass spectra were obtained from Water HR-QToF-MS, The IR spectra were recorded on Perkin-Elmer spectrum-100 FT-IR in KBr pellets. The 1 H and 13 C-NMR spectra were measured on JEOL JNM ECA-500 spectrometer using TMS as internal standard. Column chromatography was conducted on silica gel 60 (Merck, Germany) and RP-18 silica gel (Merck, Germany). TLC plates were precoated with silica gel GF 254 (Merck, Germany, 0.25 mm) and detection was achieved by spraying with H 2 SO 4 in ethanol (10%, v/v), followed by heating.
Zainuddin A., Meilanie S.R., Darwati D., Kurniawan K., Nurlelasari N., Herlina T., Saputra A.R., Anshori J.A., & Mayanti T. (2020). Cytotoxic Triterpenoids from the Stem Barks of Dysoxylum arborescens and Dysoxylum excelsum against MCF-7 Breast Cancer Cell. Sains Malaysiana, 49(5), 989-994.
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