Cytokines were quantified from supernatants of stimulated cells. IL-10 and IL-12p40 were quantified by ELISA. Cone JES5-2A5 (eBioscience) was used for IL-10 capture, and biotinylated anti-mouse IL-10 SXC-1 (BD Biosciences) was used for detection. Clone C15.6.7 was used for IL-12p40 capture, and biotinylated anti-mouse IL-12p40 C17.8 was used for detection (both gifts from DNAX Research Institute), followed by HRP-conjugated streptavidin (Jackson ImmunoResearch Laboratories). IL-12p70, TNF-α, and IL-27 (eBioscience), IL-1β (R&D Systems), and IFN-β (PBL) were quantified using commercially available ELISA kits.
Cone jes5 2a5
Manufactured by Thermo Fisher Scientific
The Cone JES5-2A5 is a laboratory equipment designed for centrifugation and separation of samples. It features a conical-shaped rotor that can accommodate various sample tubes or containers. The core function of this product is to provide a means of separating different components within a mixture based on their relative densities through the application of centrifugal force.
Automatically generated - may contain errors
2 protocols using cone jes5 2a5
1
Cytokine Quantification by ELISA
2
Cytokine Quantification from Stimulated Cells
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