Picopump pv820

Manufactured by World Precision Instruments

Sourced in United States, Japan

The PicoPump PV820 is a syringe pump designed for precise fluid delivery. It features an adjustable flow rate and can accommodate various syringe sizes. The PicoPump PV820 is suitable for a range of laboratory applications requiring accurate and controlled liquid dispensing.

Automatically generated - may contain errors

Lab products found in correlation

M 152 micromanipulator, by Narishige (2 mentions) Bf100 50 10, by Sutter Instruments (2 mentions) Micropipette puller, by Sutter Instruments (2 mentions) Ix2 slp, by Olympus (2 mentions) Phenol red, by Merck Group (1 mentions) Plasmid miniprep kit, by Evrogen (1 mentions) Phenol red dye, by Merck Group (1 mentions) Tricaine solution, by Merck Group (1 mentions) Ckx41 microscope, by Olympus (1 mentions) Xe991, by Bio-Techne (1 mentions) Wortmannin, by Merck Group (1 mentions) Pdgf bb 520 bb cf, by R&D Systems (1 mentions) Capsaicin, by Merck Group (1 mentions) Imatinib 1 5577, by LC Laboratories (1 mentions)

8 protocols using picopump pv820

Microinjection of DNA into Zebrafish Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA samples were microinjected into Danio rerio embryos at the first cleavage 20 min after fertilization using an M-152 micromanipulator (Narishige, Japan) and an air-pressure injector PicoPump PV820 (World Precision Instruments, United States) under an inverted microscope Olympus IX2-SLP (Olympus, Japan). The samples were injected into the yolk under the formed germinal disc at an angle of 45° to the surface with the embryo to maximize sample delivery into the yolk center.
The capillaries used with the outer diameter of 20 µm were pulled from glass capillaries (BF100-50–10, Sutter Instrument, United States) by a Micropipette puller (Sutter Instrument, United States). A 1 nl sample was injected into an embryo within 2.8 × 100 ms.
Vectors DNA were isolated from transformed Escherichia coli TG1 using a Plasmid Miniprep kit (Evrogen, Russia). DNA concentration was determined by spectrophotometry using the extinction coefficient of 0.02 ml/(µg × cm) for double-stranded DNA²⁷. The obtained DNA was dissolved in PBS with 0.05% phenol red (Sigma-Aldrich, United Kingdom).

Selina P.I., Karaseva M.A., Komissarov A.A., Safina D.R., Lunina N.A., Roschina M.P., Sverdlov E.D., Demidyuk I.V, & Kostrov S.V. (2021). Embryotoxic activity of 3C protease of human hepatitis A virus in developing Danio rerio embryos. Scientific Reports, 11, 18196.

+ Open protocol

+ Expand

Microinjection of DNA Samples into Zebrafish Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols

DNA samples were dissolved in PBS (Biolot, Moscow, Russia) containing 0.05% Phenol red dye (Sigma-Aldrich, St. Louis, MO, USA). DNA concentration ranged from 0.3 to 30 attomoles/nL. DNA samples were microinjected into zebrafish embryos at the first cleavage 20 min after fertilization using an M-152 micromanipulator (Narishige, Tokyo, Japan) and an air-pressure injector PicoPump PV820 (World Precision Instruments, Sarasota, FL, USA) under an inverted microscope Olympus IX2-SLP (Olympus, Tokyo, Japan). The samples (1 nL) were injected within 0.28 s into the yolk under the formed germinal disc at an angle of 45° to the plate to maximize sample delivery into the yolk center. The capillaries used with an outer diameter of 20 µm were pulled from glass capillaries (BF100-50-10, Sutter Instrument, Novato, CA, USA) by a Micropipette puller (Sutter Instrument, Novato, CA, USA). A total of 6750 eggs were injected.

Selina P.I., Alekseenko I.V., Kurtova A.I., Pleshkan V.V., Voronezhskaya E.E., Demidyuk I.V, & Kostrov S.V. (2023). Efficiency of Promoters of Human Genes FAP and CTGF at Organism Level in a Danio rerio Model. International Journal of Molecular Sciences, 24(8), 7192.

+ Open protocol

+ Expand

Neuronal Labeling in Rat Brainstem and Striatum

Check if the same lab product or an alternative is used in the 5 most similar protocols

Male and female Wistar rats (postnatal days [p] 17–21) were anesthetized with a mixture of ketamine (40 mg/kg, intramuscular injection [i.m.]) and xylazine (4 mg/kg, i.m.), followed by an injection of glycerol (0.6 g/kg, intraperitoneal injection [i.p.]) and dexamethasone (1 mg/kg, i.m.). Alexa Fluor 555-conjugated cholera toxin subunit B (CTB555, Invitrogen, Carlsbad, CA, USA) and red RetroBeads (Lumafluor, Naples, FL, USA) were injected into the pons or contralateral striatum with glass pipettes and a PicoPump (PV820, World Precision Instruments, Sarasota, FL, USA) (Morishima and Kawaguchi 2006 (link); Morishima et al. 2011 (link)).

Morishima M., Kobayashi K., Kato S., Kobayashi K, & Kawaguchi Y. (2017). Segregated Excitatory–Inhibitory Recurrent Subnetworks in Layer 5 of the Rat Frontal Cortex. Cerebral Cortex (New York, NY), 27(12), 5846-5857.

+ Open protocol

+ Expand

In utero Electroporation in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Experiments involving mice were performed in accordance with animal experimentation licenses granted to the research groups by the Landesamt für Gesundheit und Soziales, Berlin. Mice of the NMRI strain were housed, bred and operated on in the animal facility of Charité Universitätsmedizin Berlin. In utero electroporation was performed as described in (28 (link)), using a PicoPump PV820 (World Precision Instruments) and a CUY21 electroporator (Bex Co. Ltd.). Following surgery, animals were kept under observation until full recovery and the embryos collected at the pregnancy stages indicated in the experiments.

Weber A.I., Parthasarathy S., Borisova E., Epifanova E., Preußner M., Rusanova A., Ambrozkiewicz M.C., Bessa P., Newman A.G., Müller L., Schaal H., Heyd F, & Tarabykin V. (2023). Srsf1 and Elavl1 act antagonistically on neuronal fate choice in the developing neocortex by controlling TrkC receptor isoform expression. Nucleic Acids Research, 51(19), 10218-10237.

+ Open protocol

+ Expand

Xenotransplantation in Zebrafish Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols

To perform xenotransplantation, embryos at the age of 48 hpf (hours post fertilization), previously dechorionized and anesthetized with a 0.006% tricaine solution (Sigma-Aldrich, St. Louis, MO, USA), were used.
The embryos were placed onto an agarose support, and cells were injected into the yolk sac using a PicoPump PV820 pneumatic microinjector (World Precision Instruments, Inc., Sarasota, FL, USA) with the pressure parameters set at 20 psi and the sample submission time of 100 ms; an M-152 micromanipulator (Narashige Group, Tokyo, Japan); and an Olympus CKX41 microscope (Olympus, Japan). Glass capillaries (cat.# BF100-50-10 Sutter Instrument, Novato, CA, USA), with the outer diameter of 40 μm, produced by a Model P-97 device (Sutter Instrument), were employed.
The postinjection embryos were held in water at +28°C for 2 hrs, and the temperature was raised to +34 °C.
Five hours after the injections, bioimaging of the injected embryos was carried out. Individuals that showed 100–200 cells at the injection area and with no cells observed in blood vessels were selected for future analysis.

Kondratyeva L.G., Safina D.R., Chernov I.P., Kopantzev E.P., Kostrov S.V, & Sverdlov E.D. (2019). PDX1, a key factor in pancreatic embryogenesis, can exhibit antimetastatic activity in pancreatic ductal adenocarcinoma. Cancer Management and Research, 11, 7077-7087.

+ Open protocol

+ Expand

Pressure-Pulsed PDGF-BB Stimulation of DRG Neurons

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant rat carrier-free (CF) PDGF-BB (520-BB/CF, R&D Systems, Minneapolis, MN) was reconstituted to 100 µg/mL in 4 mM of HCl stock solution. Then, PDGF-BB was diluted to 125 ng/mL in extracellular solution. When indicated, PDGF-BB or its vehicle was focally and continuously applied onto plated DRG neurons using pressure puffs supplied by a pneumatic PicoPump PV820 (World Precision Instruments, Sarasota, FL), connected to a glass pipette (2-5 MΩ). The pipette was placed 25 to 50 μm away from the recorded neuron.
The vehicle solution for control experiments was 5 μΜ of HCl in the extracellular solution.
Imatinib (I-5577, LC laboratories, Woburn, MA) was dissolved in DMSO in a 10-mM stock solution. On the day of experiments, it was diluted in extracellular solution to a working concentration of 10 μM.
Wortmannin (W1628; Sigma) was dissolved with DMSO into stock aliquots. On experiment day, the stock was diluted to a working concentration of 20 nM.^{13 (link),61 (link)}XE991 (2000, Tocris Bioscience, Bristol, United Kingdom) was dissolved in water in 10-mM stock aliquots. On experiment days, the stock was diluted to a working concentration of 10 µM and applied by bath perfusion.
Capsaicin (M2028, Sigma) was applied in the bath solution at a concentration of 1 μM.

Barkai O., Puig S., Lev S., Title B., Katz B., Eli-Berchoer L., Gutstein H.B, & Binshtok A.M. (2019). Platelet-derived growth factor activates nociceptive neurons by inhibiting M-current and contributes to inflammatory pain. Pain, 160(6), 1281-1296.

+ Open protocol

+ Expand

Xenotransplantation of Panc1 Cells in Zebrafish

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Xenotransplantation of Panc1 cells transfected with siSOX9 and control siNeg was performed using Panc1-EGFP cells. Dechorionized embryos (48 h postfertilization) were injected into the yolk sac using a PicoPump PV820 pneumatic microinjector (World Precision Instruments, Inc., Sarasota, FL, USA), as previously described [27 (link)]. The bioimaging of embryos was performed 2 days after transplantation using a Leica ICC50 HD fluorescence microscope (Leica Microsystems CMS GmbH, Wetzlar, Germany) equipped with a GFP filter. Evaluation of Panc1-EGFP cell migration was performed using the digitized images of injected embryos.

Kopantzev E., Kondratyeva L., Kopantseva M., Kashkin K., Gnatenko D., Grigorieva E., Alekseenko I., Safina D, & Chernov I. (2022). SOX9 Protein in Pancreatic Cancer Regulates Multiple Cellular Networks in a Cell-Specific Manner. Biomedicines, 10(7), 1466.

+ Open protocol

+ Expand

Microinjection of OMVs into Intestinal Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Small intestinal organoids derived from Rosa26.tdTomato mice were grown for 7 days and then microinjected (n = 200/well) with purified OMVs derived from E. coli^Cre using a Pneumatic Pico Pump PV820 and a World Precision Instrument. Dynasore was added into the organoid culture medium 60 min prior to OMV microinjection in an 80 μM concentration (Dynamin Inhibitor I, Dynasore Sigma‐Aldrich).

Bittel M., Reichert P., Sarfati I., Dressel A., Leikam S., Uderhardt S., Stolzer I., Phu T.A., Ng M., Vu N.K., Tenzer S., Distler U., Wirtz S., Rothhammer V., Neurath M.F., Raffai R.L., Günther C, & Momma S. (2021). Visualizing transfer of microbial biomolecules by outer membrane vesicles in microbe‐host‐communication in vivo. Journal of Extracellular Vesicles, 10(12), e12159.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!