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Periodic acid schiff solution

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Periodic acid Schiff (PAS) solution is a reagent used in histology and cytology for the detection of carbohydrates, including polysaccharides, glycoproteins, and glycolipids, in biological samples. It is a staining procedure that involves the oxidation of these molecules by periodic acid, followed by the reaction with Schiff's reagent to produce a visible colored product.

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Lab products found in correlation

Hemavet 950 hematology system, by Drew Scientific (1 mentions) Pappenheim method, by Merck Group (1 mentions)

Spelling variants of this product

Periodic acid (89 citations) Periodic Acid-Schiff (PAS) (74 citations) Periodic acid solution (34 citations) Schiffs (2 citations)

3 protocols using periodic acid schiff solution

1

Hematology and Histopathology Analysis

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Blood parameters were determined on a Hemavet 950 hematology system (Drew Scientific), and blood smears were stained according to the Pappenheim method (Sigma-Aldrich). Tissue samples of liver, lungs, and spleen were fixed in 4% (vol/vol) formalin and embedded in paraffin. Sterni were fixed in CALfix solution (Biocyc, Luckenwalde). Deparaffinized sections were stained with hematoxylin and eosin (H&E) or periodic acid Schiff solution (Sigma-Aldrich).
Behrens K., Maul K., Tekin N., Kriebitzsch N., Indenbirken D., Prassolov V., Müller U., Serve H., Cammenga J, & Stocking C. (2017). RUNX1 cooperates with FLT3-ITD to induce leukemia. The Journal of Experimental Medicine, 214(3), 737-752.
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2

Histopathologic Analysis of Lung Inflammation

Cited 1 time
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Lung tissue was fixed in 10% (vol/vol) neutral buffered formalin and embedded in paraffin. Paraffin-embedded tissue slices were stained with H&E or periodic acid–Schiff solution (Sigma). Histopathologic analysis of inflammatory cells in H&E-stained lung sections was performed in a blinded fashion using a semi-quantitative scoring system as previously described (29 (link)).
Kim G., Lee Y., You J.S., Hwang W., Hwang J., Kim H.Y., Kim J., Jo A., Park I.H., Ali M., Kim J., Shin J.S., Kwon H.K., Kim H.J, & Yoon S.S. (2023). A Moonlighting Protein Secreted by a Nasal Microbiome Fortifies the Innate Host Defense Against Bacterial and Viral Infections. Immune Network, 23(4), e31.
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3

Kidney Injury Quantification by PAS Staining

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Paraffin-embedded kidney section was processed as mentioned followed by staining with periodic acid-Schiff solution (Sigma-Aldrich) to evaluate glomerular and renal tubular injury. At least 20 glomeruli from the kidney section were randomly selected and scored to evaluate glomerular damage. Glomerular damage index was graded as followings: 0, no sclerosis; 1, 10–25% mesangial expansion/sclerosis; 2, 25–50% mesangial expansion/sclerosis; 3, 50–75% mesangial expansion/sclerosis; 4, 75–100% mesangial expansion/sclerosis; The final score for each section was calculated as followings: Final scores = 0 × (% of grade 0 glomeruli) + 1 × (% of grade 1 glomeruli) + 2 × (% of grade 2 glomeruli) + 3 × (% of grade 3 glomeruli) + 4 × (% of grade 4 glomeruli).
Wong D.W., Yiu W.H., Chan K.W., Li Y., Li B., Lok S.W., Taketo M.M., Igarashi P., Chan L.Y., Leung J.C., Lai K.N, & Tang S.C. (2018). Activated renal tubular Wnt/β-catenin signaling triggers renal inflammation during overload proteinuria. Kidney international, 93(6), 1367-1383.
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