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2 protocols using 3 carene

1

Essential Oil Compounds Procurement

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Thymol methyl ether, Carvacrol methyl ether, (+)-2-carene, 1-octen-3-ol, myrcene, trans-anethole, α-terpineol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Carvacrol, γ-terpinene, 3-carene, p-cymene, camphene, α-terpinene, terpinolene, (+/−)-α-pinene, (−/−)-β-pinene, R-(+)-limonene, S-(−)-limonene, were purchased from Extrasynthese (Genay, France). Thymol, linalool, α-phellandrene were purchased from Merck Millipore (Boston, MA, USA). All reagents and solvents have European Pharmacopoeia quality.
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2

Antioxidant and Metal Chelation Assays

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Folin-Ciocalteu reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium peroxydisulfate, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS•+), 2,4,6-Tris(2-pyridyl)-S-triazina (TPTZ), ethylenediaminetetraacetic acid (EDTA), iron sulphate heptahydrate, ferrozine, potassium peroxydisulfate, sodium phosphate dibasic, potassium phosphate monobasic, sodium acetate, iron(III) chloride hexahydrate, iron(II) chloride tetrahydrate, and C7–C40 saturated alkane standard were purchased from Sigma-Aldrich (Milan, Italy). α-Pinene, 2-Carene, 3-Carene, β-Myrcene, and d-limonene were purchased from Extrasynthese (Lyon, France). Dichloromethane was GC-grade and was purchased from Merck (Darmstadt, Germany). All other chemicals and solvents used were of analytical grade.
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