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Ab 11212363

Manufactured by Merck Group
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AB_11212363 is a laboratory equipment product from Merck Group. It is designed for general laboratory use. The core function of this product is to provide a reliable and consistent platform for various laboratory testing and analysis procedures. Additional details about the intended use or specific applications are not available.

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Lab products found in correlation

Ab 477579, by Merck Group (2 mentions) Rat anti ha, by Merck Group (2 mentions) F7425, by Merck Group (2 mentions) Odyssey infrared imaging system, by LI COR (2 mentions)

2 protocols using ab 11212363

1

Protein Extraction and Immunoblotting in S. pombe

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extraction from S. pombe cells was done as described before (25 (link)). Membranes were probed with the required primary antibodies: rabbit anti-Flag (F7425, Sigma Aldrich), rat anti-HA (11867423001, Sigma Aldrich), mouse anti-alpha-tubulin (AB_477579, Sigma- Aldrich, 1:2500), rat anti-Ser2 Pol II (AB_11212363, Merck Millipore, 1:5000). Respective secondary antibodies, IRdye 800 and/or 680 were used to detect the signal using an Odyssey infra-red imaging system (LI-COR Biosciences).
De S., Edwards D.M., Dwivedi V., Wang J., Varsally W., Dixon H.L., Singh A.K., Owuamalam P.O., Wright M.T., Summers R.P., Hossain M.N., Price E.M., Wojewodzic M.W., Falciani F., Hodges N.J., Saponaro M., Tanaka K., Azzalin C.M., Baumann P., Hebenstreit D, & Brogna S. (2021). Genome-wide chromosomal association of Upf1 is linked to Pol II transcription in Schizosaccharomyces pombe. Nucleic Acids Research, 50(1), 350-367.
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2

Protein Extraction and Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extraction from S. pombe cells was done as described before (Matsuo et al., 2006) .
Membranes were probed with the required primary antibodies: rabbit anti FLAG (F7425, Sigma Aldrich), Rat anti-HA (11867423001, Sigma Aldrich), mouse anti-alpha-tubulin (AB_477579, Sigma-Aldrich, 1:2500), rat anti-Ser2 Pol II (AB_11212363, Merck Millipore, 1:5000). Respective secondary antibodies, IRdye 800 and/or 680 were used to detect the signal using an Odyssey infra-red imaging system (LI-COR Biosciences).
De S., Dwivedi V., Wang J., Edwards D.M., Varsally W., Singh A.K., Dixon H.L., Wojewodzic M.W., Falciani F., Hodges N.J., Saponaro M., Tanaka K., Azzalin C.M., Baumann P., Hebenstreit D., & Brogna S. (2021). Genome-wide chromosomal association of Upf1 is linked to Pol II transcription in Schizosaccharomyces pombe.
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