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Jc 10 dye

Manufactured by Abcam
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JC-10 dye is a fluorescent probe that is used to detect the mitochondrial membrane potential in live cells. It is a cationic dye that accumulates in the mitochondria in a membrane potential-dependent manner. The dye exhibits an increase in fluorescence intensity upon binding to the mitochondrial membrane.

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Lab products found in correlation

Sinergymx plate reader, by Agilent Technologies (1 mentions) Facscelesta flow cytometer, by BD (1 mentions) Facscanto, by BD (1 mentions)

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JC-10

2 protocols using jc 10 dye

1

Mitochondrial Membrane Potential Assay

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Mitochondrial membrane potential was measured using the JC-10 dye (Abcam) in BMDMs with a Sinergy Mx plate reader (BioTek) or by flow cytometry using the BD FACS Celesta flow cytometer (BD Biosciences), or in human monocytes (CD14+ labeled with anti-CD14 APC-H7, clone MφP9, catalog 560180, BD Biosciences) using FACS Canto (BD Biosciences), following the indications of manufacturer.
Martínez-García J.J., Martínez-Banaclocha H., Angosto-Bazarra D., de Torre-Minguela C., Baroja-Mazo A., Alarcón-Vila C., Martínez-Alarcón L., Amores-Iniesta J., Martín-Sánchez F., Ercole G.A., Martínez C.M., González-Lisorge A., Fernández-Pacheco J., Martínez-Gil P., Adriouch S., Koch-Nolte F., Luján J., Acosta-Villegas F., Parrilla P., García-Palenciano C, & Pelegrin P. (2019). P2X7 receptor induces mitochondrial failure in monocytes and compromises NLRP3 inflammasome activation during sepsis. Nature Communications, 10, 2711.
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2

Mitochondrial Membrane Potential Assay

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Designed positive control cells were incubated with maintenance medium containing CCCP (carbonyl cyanide m-chlorophenyl hydrazine) at 37 °C for 1 hour. All wells were then washed twice with PBS and incubated with the JC10 dye (Abcam) as per manufacturer instructions. After 45 min of incubation, the fluorescence was measured using the Infinite M-series fluorescent plate reader (TECAN) using an excitation wavelength of 490 nm and emission wavelengths of 520-570 nm.
Lazzarini E., Lodrini A.M., Arici M., Bolis S., Vagni S., Panella S., Rendon-Angel A., Saibene M., Metallo A., Torre T., Vassalli G., Ameri P., Altomare C., Rocchetti M, & Barile L. (2022). Stress-induced premature senescence is associated with a prolonged QT interval and recapitulates features of cardiac aging. Theranostics, 12(11), 5237-5257.
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