Pcr thermal cycler dice instrument

Manufactured by Takara Bio

Sourced in Japan

The PCR Thermal Cycler Dice instrument is a laboratory equipment used for the amplification of DNA samples through the Polymerase Chain Reaction (PCR) process. It precisely controls the temperature and duration of the various steps involved in the PCR reaction.

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Lab products found in correlation

Easy blue total rna extraction kit, by iNtRON Biotechnology (2 mentions) M mulv reverse transcriptase, by New England Biolabs (2 mentions) Revertaid fist strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Trizol rna extraction reagent, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Thermal Cycler Dice (131 citations) PCR Thermal Cycler Dice (50 citations) Thermal cycler (47 citations) PCR Thermal Cycler (36 citations) Thermal Cycler Dice instrument (8 citations) Thermal Cycler Dice Real-time PCR instrument (2 citations)

3 protocols using pcr thermal cycler dice instrument

Keap1/Nrf2/ARE mRNA Expression Analysis

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After treatment, the cortex was collected to determine the expression of Keap1/Nrf2/ARE mRNA by RT-PCR. Total RNA was extracted by the TRIzol RNA extraction reagent (Invitrogen, Carlsbad, CA, USA) following the protocols and quantified by spectrophotometer method. Purified RNA with equal volume was reverse transcribed (RevertAid Fist Strand cDNA Synthesis kit, K1622; Thermo Fisher Scientific, Waltham, MA, USA). RT-PCR analysis was performed using a PCR Thermal Cycler Dice instrument (Takara Bio, Inc., Otsu, Japan).

Shi L., Li H, & Zhan Y. (2017). All-trans retinoic acid enhances temozolomide-induced autophagy in human glioma cells U251 via targeting Keap1/Nrf2/ARE signaling pathway. Oncology Letters, 14(3), 2709-2714.

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RT-PCR Analysis of Apoptosis Regulators

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The cells treated with H9 were harvested. RNA was extracted using an easy-BLUE Total RNA Extraction Kit (iNtRon Biotechnology, SungNam, Korea) according to the manufacturer's instructions, as previously reported [1] . The cDNA products were obtained using M-MuLV reverse transcriptase (New England Biolabs, Beverly, MA, USA). Using a PCR Thermal Cycler Dice instrument (TaKaRa, Otsu, Shiga, Japan), we performed a RT-PCR analysis with the following primer sets: Fas 5'-AGG GAT TGG AAT TGA GGA AG-3' (forward), 5'-ATG GGC TTT GTC TGT GTA CT-3' (reverse); FasL 5'-AGT CCA CCC CCT GAA AAA AA-3' (forward), 5'-ATT CCA TAG GTG TCT TCC CA-3' (reverse); FADD 5'-ACC TCT TCT CCA TGC TG-3' (forward), 5'-CAC ACA GGT CTT CCC CA-3' (reverse); DR5 5'-GTC TGC TCT GAT CAC CCA AC-3' (forward), 5'-CTG CAA ACT GTG ACT CCT AT-3' (reverse); TRAIL 5'-GTC TCT CTG TGT GGC TGT AA-3' (forward), 5'-TGT TGC TTC TTC CTC TGG CT-3' (reverse); and GAPDH 5'-GGC TGC TTT TAA CTC TGG TA-3' (forward), 5'-TGG AAG ATG GTG ATG GGA TT-3' (reverse). GAPDH was used as an internal control.

Kwon S.B., Kim M.J., Ham S.Y., Park G.W., Choi K.D., Jung S.H, & Yoon D.Y. (2015). H9 induces apoptosis via the intrinsic pathway in non-small-cell lung cancer A549 cells. Journal of microbiology and biotechnology, 25(3).

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RT-PCR Analysis of Apoptosis-Related Genes

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After RNA extraction using an easy-BLUE total RNA extraction kit (iNtRon Biotechnology, Seoul, Korea), the cDNA products were obtained using M-MuLV reverse transcriptase (New England Biolabs, Beverly, MA, USA). We performed RT-PCR analysis using a PCR thermal cycler Dice instrument (TaKaRa, Otsu, Shiga, Japan) with the following primer sets; E6: 5'-GCAGCCCTTGAATTA CCCAT-3' (forward), 5'-CAGAGGTTGGACAGGGAAGAA-3' (reverse); E7: 5'-ATGCATGGAGATACACCTACATTGC-3' (forward), 5'-TTATGGTTTCTGAGAACAGATGGGGC-3' (reverse); Fas: 5'-TGAAGGACATGGCTTAGAAGTG-3' (forward), 5'-GGTGCAAGG GTCACAGTGTT-3' (reverse); FADD: 5'-ACCTCTTCTCCATGCTG-3' (forward), 5'-CACACAGGTCTTCCCCA-3' (reverse); DR5: 5'-GTCTGCTCTGATCACCCAAC-3' (forward), 5'-CTGCAAACT GTGACTCCTATG-3' (reverse); TRAIL: 5'-GTCTCTCTGTGTGGC TGTAA-3' (forward), 5'-TGTTGCTTCTTCCTCTGGCT-3' (reverse); and FasL: 5'-CAAGATTGACCCCGGAAGTA-3' (forward), 5'-GGC CTGTGTCTCCTTGRGAT-3' (reverse).

Hyun S., Kim M.S., Song Y.S., Bak Y., Ham S.Y., Lee D.H., Hong J, & Yoon D.Y. (2015). Peroxisome proliferator-activated receptor-gamma agonist 4-O-methylhonokiol induces apoptosis by triggering the intrinsic apoptosis pathway and inhibiting the PI3K/Akt survival pathway in SiHa human cervical cancer cells. Journal of microbiology and biotechnology, 25(3).

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