Chitosan, fucoidan, and 2′,7′-dichlorofluorescein diacetate (DCF-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Piperlongumine was supplied from Cayman Chemical (Ann Arbor, MI, USA). hDFB human dermal fibroblast cells and PC-3 human prostate cancer cells were obtained from ATCC (Manassas, VA, USA). The AnnexinV-FITC/PI staining kit was purchased from BD Life Sciences (San Jose, CA, USA). Tween 80 was purchased from Duksan Pure Chemicals (Ansan-si, Gyunggido, Korea).
Tween 80
Manufactured by Duksan Pure Chemicals
Tween 80 is a non-ionic surfactant commonly used in laboratory settings. It is a polyoxyethylene sorbitan monooleate compound that acts as a dispersing and emulsifying agent. Tween 80 is soluble in water and can be used to improve the solubility and stability of various substances in aqueous solutions.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Piperlongumine, by Cayman Chemical (1 mentions)
Fucoidan, by Merck Group (1 mentions)
Pc 3 human prostate cancer cell, by American Type Culture Collection (1 mentions)
Annexin 5 fitc pi staining kit, by BD (1 mentions)
Chitosan, by Merck Group (1 mentions)
2 7 dichlorofluorescein diacetate dcf da, by Merck Group (1 mentions)
L α lecithin, by Merck Group (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay mts kit, by Promega (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Salmon calcitonin sct, by Bachem (1 mentions)
Sct elisa kit, by Phoenix Pharmaceuticals (1 mentions)
Snakeskindialysis tubing 3.5k mwco, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 nhs ester, by Thermo Fisher Scientific (1 mentions)
Cetostearyl alcohol, by Merck Group (1 mentions)
Cetyl alcohol, by Merck Group (1 mentions)
Labrafac cc, by Gattefosse (1 mentions)
Propylene glycol, by Duksan Pure Chemicals (1 mentions)
3 protocols using tween 80
1
Apoptosis Assay of Fibroblasts and Cancer Cells
2
Salmon Calcitonin Delivery Using Cys-Penetratin
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Salmon calcitonin (sCT) was purchased from Bachem AG (Bubendorf, Switzerland). The peptide CRQIKIWFQNRRMKWKK (Cys-penetratin) was synthesized and purchased from Peptron Co., Ltd. (Daejeon, Republic of Korea). The lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl) butyramide] (MPB-PE) was purchased from Avanti Polar Lipids (Alabaster, AL). L-α-lecithin (97.7% phosphatidylcholine) was purchased from Merck Millipore (Billerica, MA, USA). Tween 80® was purchased from Duksan (Ansan, Republic of Korea). An sCT ELISA kit was purchased from Phoenix Pharmaceuticals (Burlingame, CA, USA). A Ham-F-12 Nutrient mixture (Ham’s F-12) was purchased from Welgene (Gyeongsan-si, Republic of Korea). A CellTiter 96® Aqueous One Solution Cell Proliferation Assay kit (MTS) was purchased from Promega (Madison, WI, USA). Alexa Fluor 647 NHS Ester, SnakeSkinTM dialysis tubing (3.5 K MWCO), fetal bovine serum (FBS), 0.25% trypsin-EDTA, and penicillin/streptomycin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals and solvents were of reagent grade.
3
Detailed Protocol for Lipid-Based Formulations
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ITZ, cetyl alcohol, and cetostearyl alcohol were purchased from Sigma-Aldrich Company (St. Louis, MO, USA). Labrafac® CC (caprylic/capric triglyceride) and glyceryl monostearate were obtained from Gattefosse (Lyon, France). Tween® 80 (polyoxyethylene sorbitan monooleate), Tween® 40 (polyoxyethylene sorbitan monopalmitate), Span® 60 (sorbitan monostearate), Span® 80 (sorbitan monooleate), stearic acid, paraffin oil, mineral oil, and propylene glycol were purchased from Duksan Pure Chemicals Co., Ltd. (Seoul, Korea). Polyglyceryl-3 methylglucose distearate was procured from Goldschmidt (Essen, Germany). Acetonitrile and methanol of high-performance liquid chromatography (HPLC) grade were purchased from J.T. Baker Chemical Company (Phillipsburg, NJ, USA). Distilled and deionized water was used to prepare all the solutions.
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