Jetstream 2 plus

Rapeseed oil (200 mg) was dissolved in n-hexane, made up to 10 ml, and transferred to vials for analysis. Tocopherols were qualitatively and quantitatively identified using a Waters HPLC system (Waters, Milford, MA) consisting of a pump (Waters 600), a fluorimetric detector (Waters 474), a photodiode array detector (Waters 2998 PDA), an autosampler (Waters 2707), a column oven (Waters Jetstream 2 Plus), and a LiChrosorb Si 60 column (250 × 4.6 mm, 5 µm) from Merck (Darmstadt, Germany). The mobile phase was a mixture of n-hexane with 1,4-dioxane (96:4 v/v). The flow rates were 1.0 ml/min (for tocopherols and PC-8) and 2.0 ml/min (for canolol). To detect the fluorescence of tocopherols and PC-8, the excitation wavelength was set at λ = 295 nm and the emission wavelength at λ = 330 nm. To detect the fluorescence of canolol, the excitation wavelength was set at λ = 280 nm and the emission wavelength at λ = 325 nm. Standards of α-, β-, γ-and δ-tocopherols (>95% purity) were purchased from Merck (Darmstadt, Germany). The plastochromanol-8 contents were assayed and calculated according to Siger et al. (2014) .

Rapeseed oil (200 mg) was dissolved in n-hexane, made up to 5 ml in a volumetric flask and transferred to vials for analysis. Tocopherols were qualitatively and quantitatively determined using a Waters HPLC system (Waters, Milford, MA) consisting of a pump (Waters 600), a fluorimetric detector (Waters 474) and a photo-diode array detector (Waters 2998 PDA), an autosampler (Waters 2707), a column oven (Waters Jetstream 2 Plus) and a LiChrosorb Si 60 column (250 × 4.6 mm, 5 µm) from Merck (Darmstadt, Germany). The mobile phase had a mixture of n-hexane with 1,4-dioxane (96:4 v/v). The flow rate was 1.0 ml/min. For the fluorescence detection of tocopherols and PC-8, the excitation wavelength was set at λ = 295 nm and the emission at λ = 330 nm respectively. The content of plastochromanol-8 was assayed and calculated according to Siger et al. (2014) (link).