Distearoylphosphatidylcholine dspc

Ultrapure water Milli-Q grade (MilliporeSigma, St. Louis, MO, USA) and spectroscopic grade solvents were used in all the synthesis procedures. pH buffer stock solutions in the pH range between 2 and 11 were prepared from a sodium phosphate 0.1 M solution, adjusting the pH by mixing properly a solution containing citric acid (0.05 M) and boric acid (0.2 M) in ultrapure water (all reagents from Sigma-Aldrich, St. Louis, MO, USA), following [30 (link)]. The pH values were measured with a mini-pH meter NiCd-1 (Mettler-Toledo, Greifensee, Switzerland) and the pH of each solution was properly adjusted using a solution of HCl and NaOH (1 M) (all reagents from Sigma-Aldrich, St. Louis, MO, USA). For magnetoliposomes preparation, the lipids dipalmitoylphosphatidylcholine (DPPC) (from Sigma-Aldrich, St. Louis, MO, USA), distearoylphosphatidylcholine (DSPC) (from Sigma-Aldrich, St. Louis, MO, USA), dioleoylphosphatidylethanolamine (DOPE) (from Sigma-Aldrich, St. Louis, MO, USA), cholesteryl hemisuccinate (CHEMS) (from Sigma-Aldrich, St. Louis, MO, USA) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000, from Avanti Polar Lipids, Birmingham, AL, USA) were used.

Ovalbumin (OVA), cholesterol and distearoyl phosphatidylcholine (DSPC) were acquired from Sigma-Aldrich, Inc. (St. Louis, MO). Lipidoid (C12-200) was purchased from Xinjiahecheng Medical Chemistry Corporation (Hubei, China). 1,2-Dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (mPEG-DMG) was purchased from NOF Corporation (Tokyo, Japan). The Giemsa staining kit for BALF was purchased from Baso Biotechnology Corporation (Guangdong, China).
Antibodies against F4/80 and CD206 were purchased from Santa Cruz Biotechnology (CA, USA). Antibodies against MBD2 and Arginase-1 were purchased from Abcam (MA, USA). The anti-GAPDH antibody was purchased from Proteintech (Hubei, China), while anti-Ym1 antibody was ordered from Thermo Fisher Scientific (PA, USA). PE-conjugated anti-human CD14, PE-conjugated anti-mouse F4/80, and APC-conjugated anti-mouse CD11c antibodies, ELISA kits for IL-4 and TNF-α were purchased from BioLegend (CA, USA).