Luciferin luciferase based atp determination kit

Animal serum amylase and lipase activities were determined by Antech Diagnostics (Irvine, CA) Custom Service. Active trypsin in pancreatic tissue homogenates was measured using Boc-Gln-Ala-Arg-AMC (Bachem Americas Inc, Torrance, CA) as a substrate by a fluorogenic assay as described previously [31 (link),32 (link)]. ATP level in pancreatic tissue extracts was measured according to the manufacturer’s instructions using luciferin/luciferase-based ATP determination kit (#A22066, Thermo Fisher Scientific, Grand Island, NY).

Animal serum amylase and lipase activities were determined by Antech Diagnostics (Irvine, CA) Custom Service. Amylase activity in isolated pancreatic acini and the incubation medium was determined by using a commercial kit (Phaebadas kit; Pharmacia Diagnostic, Rochester, NY) as described [31 (link)]. Amylase secretion was calculated as the percent total cellular amylase released into the medium [medium / (medium + cells)]. Caspase 3 activities in pancreatic tissue homogenates were measured using a fluorogenic assay with substrates specific for caspase-3 (Ac-DEVDAMC) as we described previously [25 (link),26 (link)]. Active trypsin in pancreatic tissue homogenates was measured using Boc-Gln-Ala-Arg-AMC (Bachem Americas Inc, Torrance, CA) as a substrate by a fluorogenic assay as described previously [31 (link),42 (link)]. Cathepsin B activities in pancreatic homogenates were measured as described previously [31 (link)] using fluorogenic cathepsin B substrate III: Z-Arg-Arg-AMC, 2HCl (Millipore Sigma, Burlington, Massachusetts). ATP level in pancreatic tissue extracts was measured according to the manufacturer’s instructions using luciferin/luciferase-based ATP determination kit (#A22066, Thermo Fisher Scientific, Grand Island, NY).