Hematoxylin and eosin

For morphologic examination, the colonic tissue sections were stained with hematoxylin and eosin (H&E) and evaluated under a light microscope (Olympus, Tokyo, Japan), according to the previously described method [15 (link), 16 (link)]. The sections were stained with Mayer's hematoxylin (Sigma-Aldrich, St. Louis, MO, USA) for 30 sec, washed, and stained with eosin (Sigma-Aldrich) for an additional 3 sec. Following dehydration, the slides were mounted using Permount® (Fisher Scientific, Fair Lawn, NJ, USA). Ischemic colonic damage was evaluated with the Wallace microscopic score (Table 1) [21 (link)].

The organs were fixed in 10% neutral buffered formalin and embedded in paraffin. Formalin-fixed and paraffin-embedded tissue slices of 3 to 5 µm were produced. Deparaffinized tissue sections were stained with hematoxylin and eosin (C.V. Laboratories, Thailand) for the histological study. Evaluation of liver morphology was initially performed with hematoxylin and eosin-stained (H&E) sections as well as other organs, including the heart, lung, kidney, small and large intestines, testes, and bone marrow of the femur, by using an Olympus BX53 microscope with a DP26 digital camera (Olympus, Japan). Based on H&E sections, hepatocellular carcinoma (HCC) was diagnosed in the presence of enlarged neoplastic hepatocytes with a high N/C ratio, nuclear pleomorphism, and eosinophilic cytoplasm that arrange in a thick trabecular or solid pattern and absence of a lobular pattern in the liver mass^{156 (link)}.