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Pierce chip kit

Manufactured by Thermo Fisher Scientific
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The Pierce ChIP Kit is a laboratory equipment product designed for chromatin immunoprecipitation (ChIP) experiments. It provides the necessary reagents and protocols to isolate and purify DNA-protein complexes from cells or tissues for further analysis.

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Lab products found in correlation

Bmal1, by Cell Signaling Technology (2 mentions) Stat1, by Cell Signaling Technology (2 mentions) Hif 1α, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

Pierce Agarose ChIP Kit (249 citations) Pierce Magnetic ChIP Kit (175 citations) ChIP kit (45 citations) Pierce™ magnetic ChIP assay kit (3 citations) Pierce Sepharose ChIP kit (2 citations) Pierce Agarose Thermo ChIP Kit (2 citations) Pierce Agarose ChIP Assay Kit (2 citations)

3 protocols using pierce chip kit

1

ChIP Assay for Transcription Factor Binding

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ChIP assay was performed using the Pierce ChIP Kit (no. 26156; Thermo Scientific). One-twentieth of the immunoprecipitated DNA was used in qPCR. Results were shown as percentage of input. BMAL1, STAT1, PKM2, and HIF1α antibodies used for ChIP were acquired from Cell Signaling Technology or Abcam. The following primers were used for qPCR of DNA quantification: Pd-l1 promoter: 5’-CACTGGCTCCTGAGTACTGG-3’ and 5’-CTGTCTGTGAAACCGAAGCC-3’; Pkm2 promoter: 5’-TGGTCTACGATGTCCTTCCG-3’ and 5’-TGAGAGAAGCTCAGGGTAGG-3’.
Deng W., Zhu S., Zeng L., Liu J., Kang R., Yang M., Cao L., Wang H., Billiar T.R., Jiang J., Xie M, & Tang D. (2018). The Circadian Clock Controls Immune Checkpoint Pathway in Sepsis. Cell reports, 24(2), 366-378.
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2

ARNTL ChIP Assay Protocol

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A ChIP assay was performed using the Pierce ChIP Kit (no. 26156, Thermo Fisher Scientific) according to the manufacturer’s guidelines. One-twentieth of the immunoprecipitated DNA was used in qPCR. Results were shown as a percentage of input. ARNTL antibody (no. 14020) used for ChIP was acquired from Cell Signaling Technology.
Yang M., Chen P., Liu J., Zhu S., Kroemer G., Klionsky D.J., Lotze M.T., Zeh H.J., Kang R, & Tang D. (2019). Clockophagy is a novel selective autophagy process favoring ferroptosis. Science Advances, 5(7), eaaw2238.
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3

ChIP Assay for Transcription Factor Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assay was performed using the Pierce ChIP Kit (no. 26156; Thermo Scientific). One-twentieth of the immunoprecipitated DNA was used in qPCR. Results were shown as percentage of input. BMAL1, STAT1, PKM2, and HIF1α antibodies used for ChIP were acquired from Cell Signaling Technology or Abcam. The following primers were used for qPCR of DNA quantification: Pd-l1 promoter: 5’-CACTGGCTCCTGAGTACTGG-3’ and 5’-CTGTCTGTGAAACCGAAGCC-3’; Pkm2 promoter: 5’-TGGTCTACGATGTCCTTCCG-3’ and 5’-TGAGAGAAGCTCAGGGTAGG-3’.
Deng W., Zhu S., Zeng L., Liu J., Kang R., Yang M., Cao L., Wang H., Billiar T.R., Jiang J., Xie M, & Tang D. (2018). The Circadian Clock Controls Immune Checkpoint Pathway in Sepsis. Cell reports, 24(2), 366-378.
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