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2 protocols using phosphate buffered saline preformulated tablets

1

Glycan Engineering via Metabolic Labeling

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Ammonium fluoroborate (NH4BF4), dodecanethiol, tripotassium phosphate (K3PO4), 2-bromo-2-methylpropionic acid, dichloromethane (DCM), hydrochloric acid (HCl), magnesium sulfate (MgSO4), hexane, 4-(dimethylamino) pyridine, N-(3-(dimethylamino)propyl)-N′-ethylcarbodiimide hydrochloride, penta-fluorophenol (PFP), sodium bicarbonate (NaHCO3), sodium chloride (NaCl), N-hydroxyethyl acrylamide (HEA), 4,4′-azobis(4-cyanovaleric acid) (ACVA), toluene, methanol, mesitylene, dibenzocyclooctyne-amine (DBCO-NH2), propyl amine, paraformaldehyde, phosphate-buffered saline preformulated tablets, dimethylformamide, N-(5-fluoresceinyl)maleimide, calcium chloride (CaCl), and magnesium chloride (MgCl) were purchased from Sigma-Aldrich Co Ltd. (Gillingham, UK) and used without further purification. Dulbecco phosphate buffered saline (DPBS), N-azidoacetylmannosamine-tetraacylated (Ac4ManNAz), and BD FACSFlow Sheath Fluid were purchased from Fisher Scientific (Loughborough, UK). α2-3,6,8,9 Neuraminidase A (316 000 units·mg−1) was purchased from New England BioLabs (UK) LTD (Hitchin).
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2

Glycan Engineering via Metabolic Labeling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ammonium fluoroborate (NH4BF4), dodecanethiol, tripotassium phosphate (K3PO4), 2-bromo-2-methylpropionic acid, dichloromethane (DCM), hydrochloric acid (HCl), magnesium sulfate (MgSO4), hexane, 4-(dimethylamino) pyridine, N-(3-(dimethylamino)propyl)-N′-ethylcarbodiimide hydrochloride, penta-fluorophenol (PFP), sodium bicarbonate (NaHCO3), sodium chloride (NaCl), N-hydroxyethyl acrylamide (HEA), 4,4′-azobis(4-cyanovaleric acid) (ACVA), toluene, methanol, mesitylene, dibenzocyclooctyne-amine (DBCO-NH2), propyl amine, paraformaldehyde, phosphate-buffered saline preformulated tablets, dimethylformamide, N-(5-fluoresceinyl)maleimide, calcium chloride (CaCl), and magnesium chloride (MgCl) were purchased from Sigma-Aldrich Co Ltd. (Gillingham, UK) and used without further purification. Dulbecco phosphate buffered saline (DPBS), N-azidoacetylmannosamine-tetraacylated (Ac4ManNAz), and BD FACSFlow Sheath Fluid were purchased from Fisher Scientific (Loughborough, UK). α2-3,6,8,9 Neuraminidase A (316 000 units·mg−1) was purchased from New England BioLabs (UK) LTD (Hitchin).
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