Primary rabbit polyclonal antibody to caveolin 1

Tissue sections (4 μm) were prepared from formalin-fixed paraffin-embedded blocks. Immunostaining was done according to our laboratory method.[21 (link)] Briefly, the deparaffinised section was quenched with 3% H₂O₂ for 10 min. After washing with phosphate buffered saline (PBS), the sections were incubated in 5% bovine serum albumin for 20 min, followed by incubation with primary rabbit polyclonal antibody to caveolin-1 (1:50) (Santa Cruz Biotechnology, Inc, USA.) for 60 min at 37^° C and secondary antibodies for 30 min at room temperature. The localisation of caveolin-1 was visualised by 3,3 diaminobenzidine (DAB) solution for 3-5 min. Finally, the slides were washed with water before being counterstained with haematoxylin. PBS was substituted for primary antibody as a negative control.

Phosphate buffered saline (PBS) was purchased from Hyclone Reagent Co, Ltd, Beijing, China. Anti-rabbit/mouse polymer, Fresh DAB (3,3-diaminobenzidine) was purchased from Zhongshan Golden Bridge Reagent Co, Ltd, Beijing, China. Primary rabbit polyclonal antibody to caveolin-1 was purchased from Santa Cruz Biotechnology, Inc, USA.