Ruxolitinib s1378

Pentobarbital sodium was purchased from Amresco (Cleveland, OH, USA). Krebs-Henseleit Bicarbonate Buffer was purchased from M&C GENE Technology (Beijing) Ltd. (Beijing, China). anti-IL-6 monoclonal antibody (anti-IL-6 mAb) for rats was purchased from InvivoGen (CA, USA). JAK1/2 inhibitor ruxolitinib (S1378) was purchased from Selleck Chemicals (Houston, TX). The primary antibodies including the anti-IL-6, anti-p-JAK1/2, anti-JAK1/2, anti-p-STAT3/5, anti-STAT3/5, and anti-LC3B-I/II were purchased form Abcam (Shanghai, China). MitoSOX™ Red Mitochondrial Superoxide Indicator was purchased from Invitrogen (CA, USA). Myosin Heavy Chain (NOQ7.5.4D) (ab11083), anti-Fast Myosin Skeletal Heavy Chain (MY-32) (ab51263), and anti-laminin were purchased from Abcam (Shanghai, China). Hydrogen Peroxide Assay Kit was purchased from BioVision (Palo Alto, CA, USA). Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α (Cat no. RTA00), IL-1β (Cat no. RLB00), and IL-6 (Cat no. R6000B) were purchased from R&D Systems Inc. (Minneapolis, MN, USA). BCA protein assay kit was purchased from Beyotime (Shanghai, China). Lactate Assay Kit was purchased from BioVision (CA, USA).