Duel endogenous enzyme block

Manufactured by Agilent Technologies

The Duel Endogenous Enzyme Block is a laboratory tool designed to inhibit the activity of endogenous enzymes in biological samples. It functions by blocking the catalytic action of these enzymes, which can otherwise interfere with experimental results.

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Lab products found in correlation

Jc 70a, by Abcam (1 mentions) Epr23097 35, by Abcam (1 mentions)

2 protocols using duel endogenous enzyme block

Immunohistochemistry of SARS-CoV-2 Spike Protein

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The tissue slides were deparaffinized, rehydrated, and blocked with Duel Endogenous Enzyme Block (Dako). Tissue sections were probed with the primary antibody against fibrin (59D8; Millipore), CD42b (SP219; Abcam; RRID: AB_2814749), VWF (F8/86; Invitrogen; RRID: AB_11001165), PECAM-1 (JC/70A; Abcam; RRID: AB_307284), or SARS-CoV-2 spike S1 protein (007; Sino Biological; AB_2827979) and then treated with HRP-conjugated secondary goat anti-rabbit or anti-mouse IgG antibody (Leica). The slides were counterstained with 50% hematoxylin in water.

Won T., Wood M.K., Hughes D.M., Talor M.V., Ma Z., Schneider J., Skinner J.T., Asady B., Goerlich E., Halushka M.K., Hays A.G., Kim D.H., Parikh C.R., Rosenberg A.Z., Coppens I., Johns R.A., Gilotra N.A., Hooper J.E., Pekosz A, & Čiháková D. (2022). Endothelial thrombomodulin downregulation caused by hypoxia contributes to severe infiltration and coagulopathy in COVID-19 patient lungs. EBioMedicine, 75, 103812.

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Immunohistochemical Staining of Tissue Sections

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The biopsy tissue slides were deparaffinized, rehydrated, and blocked with Duel Endogenous Enzyme Block (Dako). Tissue sections were probed with primary antibody against CD68 (KP1, Abcam) or CD3 (SP7, Abcam) and then treated with HRP-conjugated secondary goat antibody against rabbit or mouse IgG (Leica). Mouse heart sections were stained with anti-NCR1 antibody (EPR23097-35, Abcam). The slides were counterstained with 50% hematoxylin in water.

Won T., Gilotra N.A., Wood M.K., Hughes D.M., Talor M.V., Lovell J., Milstone A.M., Steenbergen C, & Čiháková D. (2022). Increased Interleukin 18-Dependent Immune Responses Are Associated With Myopericarditis After COVID-19 mRNA Vaccination. Frontiers in Immunology, 13, 851620.

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