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Af2720

Manufactured by R&D Systems
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The AF2720 is a laboratory equipment product. It is designed to perform core functionality for research and development applications. No further details on its intended use or specific features are provided.

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Lab products found in correlation

Af762, by R&D Systems (3 mentions) Sc 1616, by Santa Cruz Biotechnology (1 mentions) Ab28364, by R&D Systems (1 mentions) Af2727, by R&D Systems (1 mentions) Alexa fluor 568, by Jackson ImmunoResearch (1 mentions) Ap192c, by Merck Group (1 mentions) Af1002, by R&D Systems (1 mentions) Vwf a0082, by Agilent Technologies (1 mentions) Alexa fluor 488, by Jackson ImmunoResearch (1 mentions) Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions) Alexa fluor 647, by Jackson ImmunoResearch (1 mentions)

3 protocols using af2720

1

Phosphorylated TIE2 Expression in Lung Carcinoma

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Lewis lung carcinoma tumor‐bearing mice were treated with either AB‐Tie1‐39 or IgG, and on day 14, lung tissues were snap‐frozen for analysis. Subsequent to RIPA‐based lysis, 50 μg tissue lysates were loaded on SDS–PAGE. Transferred blots were probed with anti‐phospho‐TIE2 (Y992; AF2720, R&D Systems, 1:1,000), anti‐total‐TIE2 (AF762, R&D Systems, 1:1,000), and anti‐ACTB (sc‐1616, Santa Cruz Biotechnology, 1:1,000). Densitometric quantification of pTIE2 and tTIE2 was performed using FIJI software.
Singhal M., Gengenbacher N., La Porta S., Gehrs S., Shi J., Kamiyama M., Bodenmiller D.M., Fischl A., Schieb B., Besemfelder E., Chintharlapalli S, & Augustin H.G. (2020). Preclinical validation of a novel metastasis‐inhibiting Tie1 function‐blocking antibody. EMBO Molecular Medicine, 12(6), e11164.
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2

Immunofluorescence Staining of VE-cadherin and Tie2

Cited 2 times
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The following primary antibodies were used for immunofluorescence staining at the indicated concentrations: VE-PTP (rat-anti-mouse-VE-PTP clone 109.1, 10 µg/mL),33 (link) (rabbit-anti-human VE-PTP, hVE-PTP 1-8 antibodies were generated in rabbits against a recombinant form of the extracellular fibronectin type III-like domains 1-8 of human VE-PTP and affinity purified using the antigen), VE-cadherin (rabbit-a-mouse-VE-cadherin, pAB42)35 (link) Prox1 (goat-a-human-Prox1, R&D, AF-2727, 2 µg/mL); PECAM-1 (rat-a-mouse PECAM-1 clone 1 G5.1 (3 µg/mL) + clone 5D2.6 (1 µg/mL),36 (link) (rabbit polyclonal anti-PECAM-1, Abcam, ab28364); Tie2 (goat-a-mouse-Tie2, R&D, AF762, 5 µg/mL); and phospho-Tie2 (rabbit-a-pY992-Tie2, R&D, AF2720, 10 µg/mL). The following Alexa Fluor labeled secondary antibodies (all from Thermo Fisher Scientific) were used at a concentration of 2 µg/mL: donkey-a-rat-IgG-Alexa-488 (A-21208); donkey-a-goat-IgG-Alexa-568 (A-11057); donkey-a-goat-IgG-Alexa-647 (A-10042); and donkey-a-rabbit-IgG-Alexa-568 (A-21447).
Li G., Nottebaum A.F., Brigell M., Navarro I.D., Ipe U., Mishra S., Gomez-Caraballo M., Schmitt H., Soldo B., Pakola S., Withers B., Peters K.G., Vestweber D, & Stamer W.D. (2020). A Small Molecule Inhibitor of VE-PTP Activates Tie2 in Schlemm's Canal Increasing Outflow Facility and Reducing Intraocular Pressure. Investigative Ophthalmology & Visual Science, 61(14), 12.
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3

Immunohistochemical Staining of Vascular Markers

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Primary antibodies used for immunohistochemical staining included: rat monoclonal anti-mouse VE-PTP (clone 109.1, 1:500, (Baumer et al. 2006 (link))); goat polyclonal anti-mouse Tie2 (AF762, 1:500, R&D Systems); rabbit polyclonal tyrosine-992 phosphorylated anti-mouse Tie2 (Tie2-pY992, AF2720, 1:500, R&D Systems); rabbit polyclonal anti-mouse claudin-5 (34-1600, 1:1000, Invitrogen); rabbit monoclonal anti-mouse VE-cadherin (clone Vii37, MABT886, 1:1000, Sigma); rat monoclonal anti-mouse VE-cadherin (BD 550548, 1:1000, BD Biosciences); goat polyclonal anti-mouse VE-cadherin (AF1002, 1:1000, R&D Systems); rabbit polyclonal anti-human von Willebrand factor (vWF, A0082, 2 μg/ml, Dako); Cy5-conjugated donkey anti-mouse IgG (AP192C, 1:500, Sigma); and chicken polyclonal anti-human lamin A/C (NBP-2-25152, 1:500, Novus). Secondary antibodies were conjugated to Cy3, Alexa Fluor 488, Alexa Fluor 568, or Alexa Fluor 647 (Jackson ImmunoResearch).
Baluk P., Shirakura K., Vestweber D, & McDonald D.M. (2023). Heterogeneity of endothelial VE-PTP downstream polarization, Tie2 activation, junctional claudin-5, and permeability in the aorta and vena cava. Cell and Tissue Research, 395(1), 81-103.
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