egg-white lysozyme (HEWL), sodium dihydrogen
phosphate dihydrate, sodium bromide, sodium hydroxide, glycine, acetic
acid, Amicon Ultra-15 centrifugal units, Spectra/Por float-a-lyzer
G2 dialysis tubes and hydrochloric acid were purchased from Merck
(Darmstadt, Germany). Cacodylic acid, HEPES, MOPS, and TRIS were obtained
from Sigma-Aldrich (St. Louis, U.S.A.). Disodium hydrogen phosphate
was purchased from Chem-Lab (Zedelgem, Belgium).