After extraction of tissue and cellular protein, protein was separated by SDS/PAGE and electro-transferred to PVDF membranes. The resulting membranes were blocked with 0.1% (w/v) BSA solution on a shaker for 1 h. Then, they were incubated with primary antibodies at 4°C overnight. The antibodies including: VDR (ab109234, Abcam), cleaved caspase-3 (ab49822, ab214430, Abcam), bcl2 (226593-1-AP, ProteinTech), PDHA1 (sc-377092, Santa Curz Biotechnology), p-PDHA1 (ab177461, Abcam), p-AMPK (2535, Cell Signaling Technology), AMPK (2532, Cell Signaling Technology), β-actin (20536-1-AP, ProteinTech), α-tublin (AF7010, Affinity). After that, the membrane was incubated with the fluorescent secondary antibody for 1 h before three times with TBST. Finally, the protein expression levels were visualized by the Image Studio software and band intensities were quantified with Image J gel analysis software.
P pdha1
Manufactured by Abcam
P-PDHA1 is a lab equipment product. It is a protein that plays a core function in cellular metabolism.
Automatically generated - may contain errors
Lab products found in correlation
P ampk, by Cell Signaling Technology (1 mentions)
β actin 20536 1 ap, by Proteintech (1 mentions)
Ab49822, by Abcam (1 mentions)
Ab214430, by Abcam (1 mentions)
Ab109234, by Abcam (1 mentions)
Ab177461, by Abcam (1 mentions)
Af7010, by Affinity Biosciences (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Mmp14, by Santa Cruz Biotechnology (1 mentions)
Tnfr1, by Abcam (1 mentions)
E cadherin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using p pdha1
1
Western Blot Analysis of Protein Targets
2
Antibody Detection and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The primary antibodies used in the present study included the following: PDHA1 (1:500; cat. no. 377092 Santa Cruz Biotechnology, Inc.), p-PDHA1 (1:1,000; no. ab115343; Abcam), TNFR1 (1:1,000; cat. no. ab68160; Abcam), MMP2 (1:500; cat. no. 13595; Santa Cruz Biotechnology, Inc.), MMP14 (1:500; cat. no. 373908; Santa Cruz Biotechnology, Inc.), E-cadherin (1:1,000; cat. no. 14472; Cell Signaling Technology, Inc.), GAPDH (1:5,000; cat. no. ab8245; Abcam).
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