Perforin dg9

Manufactured by BioLegend

Perforin (dG9) is a cytotoxic protein found in the granules of cytotoxic T cells and natural killer cells. It functions to create pores in the membranes of target cells, facilitating the delivery of granzymes and the induction of apoptosis.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using perforin dg9

Multicolor Immunohistochemistry of Brain Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Brian tissue sections were incubated overnight with primary antibodies at 4°C and then with corresponding fluorochrome-conjugated secondary antibodies at room temperature for 1 h. The following primary antibodies were used: anti-human NKp46 (195314; R&D Systems), CD49a (SR84; BD Bioscience), CD57 (MA1-81071; Invitrogen), CD68 (ED1; Abcam), CD4 (H-370; Santa Cruz), CD8 (UCH-T4; Santa Cruz), CD19 (HIB19; BioLegend), CD16b (CLB-gran11.5; BD Bioscience), CD66b (polyclonal; Bioss), perforin (dG9; BioLegend), CD69 (D-3; Santa Cruz), Caspase-3 (9661; CST), CD31 (JC/70A; Abcam); anti-mouse NKp46 (M20; Santa Cruz), CD49a (Ha31/8; BD Bioscience), ly6G (1A8; BioLegend), TMEM119 (28-3; Abcam), CD68 (ED1; Abcam), CD8 (53-6.7; eBioscience), CD4 (GK1.5; eBioscience), CD19 (1D3; BD Bioscience), CD31 (polyclonal; Abcam), claudin5 (4C3C2; Invitrogen), and ZO-1 (ZO-1-1A12; Invitrogen). The following fluorochrome-conjugated secondary antibodies were used: donkey anti-rabbit 488 (1:1,000; Invitrogen), donkey anti-rabbit 546 (1:1,000; Invitrogen), donkey anti-goat 546 (1:1,000; Invitrogen), donkey anti-mouse 594 (1:1,000; Invitrogen), donkey anti-mouse 488 (1:1,000; Invitrogen), goat anti-rat 488 (1:1,000; Invitrogen), and goat anti-rat 555 (1:1,000; Invitrogen). Images were acquired on a fluorescence microscope (Olympus BX-61).

Li Z., Li M., Shi S.X., Yao N., Cheng X., Guo A., Zhu Z., Zhang X, & Liu Q. (2020). Brain transforms natural killer cells that exacerbate brain edema after intracerebral hemorrhage. The Journal of Experimental Medicine, 217(12), e20200213.

+ Open protocol

+ Expand

Multiparametric Analysis of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMCs were surface-stained with anti-TCRα/β/TCRγ/δ/CD3//CD4/CD8/CD56 and anti-CD25 (BC96), CD45RA (HI100), CD45RO (UCHL1), CD69 (FN50) or HLA-DR (G46-6), (Biolegend, San Diego, CA). For intracellular staining, cells were fixed and permeabilized (BD Biosciences, San Jose, CA), and stained with granzyme B (GB12; Invitrogen, Grand Island, NY) or perforin (dG9; Biolegend, San Diego, CA). FoxP3 staining (206D; Biolegend, San Diego, CA) was performed as per manufacturer's recommendations (eBioscience, San Diego, CA).

Tarbox J.A., Keppel M.P., Topcagic N., Mackin C., Abdallah M.B., Baszis K.W., White A.J., French A.R, & Cooper M.A. (2014). Elevated Double Negative T Cells in Pediatric Autoimmunity. Journal of clinical immunology, 34(5), 594-599.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!