Mouse cytokine antibody array

Manufactured by Abcam

Sourced in United Kingdom

The Mouse Cytokine Antibody Array is a lab equipment product designed for the simultaneous detection and quantification of multiple mouse cytokines in a single experiment. It provides a comprehensive overview of the cytokine profile in mouse samples.

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Lab products found in correlation

Phospho akt ser473, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Amersham imager 680, by Cytiva (1 mentions) E cadherin, by GeneTex (1 mentions) Fusion fx6, by Vilber (1 mentions) Amersham imager 600 imager, by GE Healthcare (1 mentions) Supersignal west femto, by Thermo Fisher Scientific (1 mentions)

3 protocols using mouse cytokine antibody array

Cytokine Profiling and Signaling in BALF

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BALF samples were prepared from three mice in each group, pooled, and subjected to a Mouse Cytokine Antibody Array (ab193660, Abcam) according to the manufacturer’s instructions. Pooled BALF containing 100 μg of protein was used for each membrane. Signals were detected on an Amersham Imager 680 instrument (Cytiva) and analyzed by Image J.
For immunoblotting, lysates were prepared using RIPA buffer (10 mM Na-phosphate, pH 7.2, 150 mM NaCl, 2 mM EDTA, 0.1% SDS, 1% Na-deoxycholate, and 1% NP-40). Proteins were separated by SDS−PAGE, transferred to a PVDF membrane, and were detected using antibodies to E-cadherin (GTX100443, GeneTex), β-Actin (#12620, Cell Signaling Technology), phospho-Akt (ser473) (#4060, Cell Signaling Technology), and Akt (#4691, Cell Signaling Technology).

Kawaguchi K., Komoda K., Mikawa R., Asai A, & Sugimoto M. (2021). Cellular senescence promotes cancer metastasis by enhancing soluble E-cadherin production. iScience, 24(9), 103022.

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Coculture of PC12 and 4T1 Cells

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PC12 cells were cocultured with 4T1 for 72 hours. Cells were seeded in six-well plate in serum-reduced media at a density of 4 × 10⁵ cells per well for PC12 cells and at a density of 2.5 × 10⁵ cells per well for 4T1. In addition, 4T1 cells were seeded alone at a density of 3 × 10⁵ cells per well. Cytokine profile of the conditioned medium was determined using Mouse Cytokine Antibody Array (Abcam, Cambridge, UK). The membranes were imaged using FUSION FX 6 (VILBER, France). Semiquantitative data were assessed using the ROI tool in Fiji software, and the obtained values were normalized to the positive control of each membrane.

Kaduri M., Sela M., Kagan S., Poley M., Abumanhal-Masarweh H., Mora-Raimundo P., Ouro A., Dahan N., Hershkovitz D., Shklover J., Shainsky-Roitman J., Buganim Y, & Schroeder A. (2021). Targeting neurons in the tumor microenvironment with bupivacaine nanoparticles reduces breast cancer progression and metastases. Science Advances, 7(41), eabj5435.

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Profiling Intracellular Cytokines in Frozen Cells

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Live single cells that were frozen in 90%FBS+10%DMSO described in the tissue harvest section were thawed and pooled to perform the Mouse Cytokine Antibody Array (ab133993, Abcam), according to the manufacture’s protocol. Single cells were lysed and 250μg protein were used on each membrane. This assay only measured the intra-cellular cytokines as the excreted cytokines were washed off during the single cell prep. Membranes were developed by SuperSignal West Femto (34095, Thermo Scientific) and scanned on Amersham Imager 600 imagers (29083463, GE). Image was quantified by ImageJ.

Janghorban M., Yang Y., Zhao N., Hamor C., Nguyen T.M., Zhang X.H, & Rosen J.M. (2022). Single Cell Analysis Unveils the Role of the Tumor Immune Microenvironment and Notch Signaling in Dormant Minimal Residual Disease. Cancer research, 82(5), 885-899.

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