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2 protocols using 104y gal4

1

Drosophila Genetic Manipulation Toolkit

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Fly strains were maintained on a standard diet at 25 ℃, 12 light:12 dark cycles and 70% humidity. Actin-GeneSwitch (#9381), Dilp2-Gal4 (#37516), UAS-dSec16 RNAi (#53917), Ok107-Gal4 (#854), 104y-Gal4 (#81014), Dsk-Gal4 (#51981), Dh44-Gal4 (#51987), Lk-Gal4 (#51992), OK371-Gal4 (#26160), Chat-Gal4 (#6793), 55D01-Gal4 (#39110), Ppk-Gal4 (#79278) and Pdf-Gal4 (#6899) were purchased from the Bloomington Stock Center.
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2

Optogenetic Manipulation of Neuronal Circuits in Flies

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Flies were cultured on standard agar medium under a 12-hr day/night cycle. Flies used for optogenetics were placed on food containing 1 mM all-trans retinal (Sigma) for two days before experiments. All flies were outcrossed six generations to a w2202 genetic background (isoCJ1; (Yin et al., 1994 (link)). The INX6-RNAi lines were a gift from Chia-Lin Wu. UAS-CsChrimson was a gift from Vivek Jayaraman. R23E10-Gal4, C5-Gal4, 104y-Gal4, and UAS-shibire were obtained from Bloomington. The INX6 RNAi is v8638, originally obtained from the VDRC (Dietzl et al., 2007 (link)). UAS-syx3-69 was obtained from Bing Zhang.
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