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Isobutyl chlorocarbonate

Manufactured by Merck Group
Sourced in United States

Isobutyl chlorocarbonate is a laboratory reagent used in organic synthesis. It is a colorless liquid with a pungent odor. The primary function of isobutyl chlorocarbonate is as a chloroformate reagent for the introduction of isobutyloxycarbonyl (Ioc) protecting groups onto amine compounds.

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2 protocols using isobutyl chlorocarbonate

1

Development of a Multiplex Immunoassay for Pharmaceutical Screening

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CYP and loratadine, ketotifen, clenbuterol, clonidine and salbutamol (purity 99.0%) were obtained from Anpel Co., Ltd (Shanghai, China). Bovine serum albumin (BSA), dicyclohexylcarbodiimide (DCC), N,N-dimethylformamide (DMF), ovalbumin (OVA), trinbutylamine, isobutyl chlorocarbonate, N-hydroxysuccinimide (NHS), hypoxanthine-aminopterin-thymidine (HAT), complete and incomplete Freund's adjuvant, hypoxanthine-thymidine (HT) medium, peroxidase-labeled goat anti-mouse IgGs, 3,3′,5,5′-tetramethylbenzidine (TMB) and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich (St Louis, MO, USA). Nitrocellulose (NC) membranes, glass fiber sample pads and absorbent pad were purchased from Millipore Corporation (Bedford, MA, USA). Semi rigid polyvinyl chloride (PVC) sheets were obtained from Jiening Biotech (Shanghai, China). Quantum dots (QDs) were obtained in Prof. Yonghua Xiong's lab (Nanchang University, Nanchang, China). Dichloromethane (DCM), polyethylene glycol (PEG) 20000, poly (methyl methacrylate) (PMMA), poly (maleic anhydride-alt-1-octadecene) (PMAO), and other chemicals were obtained from Aladdin Chemistry Co., Ltd. (Shanghai, China).
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2

Quantitative Immunochromatographic Assay for Tebuconazole

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Tebuconazole (TEB), Ovalbumin (OVA), bovine serum albumin (BSA), N-(3-(dimethylamino)propyl)-N′-ethylcarbo-diimide hydrochloride (EDC·HCl), dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), tri-n-butylamine, isobutyl-chlorocarbonate, complete and incomplete Freund’s adjuvant, poly-ethylene glycol (PEG) 2000, hypoxanthine-aminopterin-thymidine (HAT) and hypoxanthine-thymidine (HT) medium, peroxidase-labeled goat antimouse IgGs, 3,3′,5,5′-tetramethylbenzidine (TMB), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich Chemical (St. Louis, MO, USA). Cell culture medium (DMEM) and fetal bovine serum were provided by Thermo Fisher Scientific (Waltham, MA, USA). TEB analogues (penconazole, propiconazole, myclobutanil, paclobutrazol, and hexaconazole) used for cross-reactivity studies were supplied by Aladdin Chemistry Co., Ltd. (Shanghai, China).
Quantum dot beads (QBs) were generated in Prof. Yonghua Xiong’s lab (Nanchang University, Nanchang, China).22 (link) The elements of the immunochromatographic test strip platform comprising sample, absorbent pads, and NC membranes were purchased from Millipore Corp. (Bedford, MA, USA).
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