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Bv605 conjugated ifn γ

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BV605-conjugated IFN-γ is a fluorescent-labeled antibody used for the detection and quantification of interferon-gamma (IFN-γ) in flow cytometry applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) K562 cell line, by American Type Culture Collection (2 mentions) Golgiplug protein transport inhibitor, by BD (2 mentions) Bv785 conjugated anti cd3 clone okt3, by BioLegend (2 mentions) Pe cf594 conjugated anti cd57, by BioLegend (2 mentions) Percp cy5.5 conjugated anti cd107a, by BioLegend (2 mentions) Af700 conjugated anti tnf, by BioLegend (2 mentions) Pecy7 conjugated anti cd56, by BioLegend (2 mentions) Pe conjugated nkg2c, by R&D Systems (2 mentions) Ficoll paque, by GE Healthcare (2 mentions) Golgistop, by BD (2 mentions)

Spelling variants of this product

IFN-γ (422 citations) IFN-γ-BV605 (4 citations)

2 protocols using bv605 conjugated ifn γ

1

Functional Analysis of NK Cell Subsets

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Buffy coats collected from 5 healthy CMV seropositive donors were obtained from Memorial Blood Bank (Minneapolis, MN). Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare) and cultured with K562 cells at a 2:1 (effector:target) ratio for 5 hours in RPMI media supplemented with 10% fetal bovine serum (Gibco). GolgiStop and GolgiPlug protein transport inhibitors (BD Biosciences) were added 1 hour into the assay. The following antibodies were used for functional analysis of NK cell subsets: BV785-conjugated anti-CD3 (clone OKT3; Biolegend), PECy7-conjugated anti-CD56 (BioLegend; 318318), PE-CF594-conjugated anti-CD57 (Biolegend; 359620), PE-conjugated NKG2C (R&D Systems; FAB138P-025), PerCP-Cy5.5-conjugated anti-CD107a (Biolegend; 328616), AF700-conjugated anti-TNF (Biolegend; 502928) and BV605-conjugated IFN-γ (Biolegend; 502536). The K562 cell line was purchased from ATCC (Manassas, Virginia) and is screened monthly for mycoplasma contamination. The experiment was performed at 2 independent times. Two-sided, paired t-tests in GraphPad were used to determine significance. Error bars represent SEM.
Cichocki F., Cooley S., Davis Z., DeFor T.E., Schlums H., Zhang B., Brunstein C.G., Blazar B.R., Wagner J., Diamond D.J., Verneris M.R., Bryceson Y.T., Weisdorf D.J, & Miller J.S. (2015). CD56dimCD57+NKG2C+ NK cell expansion is associated with reduced leukemia relapse after reduced intensity HCT. Leukemia, 30(2), 456-463.
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2

Functional Analysis of NK Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Buffy coats collected from 5 healthy CMV seropositive donors were obtained from Memorial Blood Bank (Minneapolis, MN). Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using Ficoll-Paque (GE Healthcare) and cultured with K562 cells at a 2:1 (effector:target) ratio for 5 hours in RPMI media supplemented with 10% fetal bovine serum (Gibco). GolgiStop and GolgiPlug protein transport inhibitors (BD Biosciences) were added 1 hour into the assay. The following antibodies were used for functional analysis of NK cell subsets: BV785-conjugated anti-CD3 (clone OKT3; Biolegend), PECy7-conjugated anti-CD56 (BioLegend; 318318), PE-CF594-conjugated anti-CD57 (Biolegend; 359620), PE-conjugated NKG2C (R&D Systems; FAB138P-025), PerCP-Cy5.5-conjugated anti-CD107a (Biolegend; 328616), AF700-conjugated anti-TNF (Biolegend; 502928) and BV605-conjugated IFN-γ (Biolegend; 502536). The K562 cell line was purchased from ATCC (Manassas, Virginia) and is screened monthly for mycoplasma contamination. The experiment was performed at 2 independent times. Two-sided, paired t-tests in GraphPad were used to determine significance. Error bars represent SEM.
Cichocki F., Cooley S., Davis Z., DeFor T.E., Schlums H., Zhang B., Brunstein C.G., Blazar B.R., Wagner J., Diamond D.J., Verneris M.R., Bryceson Y.T., Weisdorf D.J, & Miller J.S. (2015). CD56dimCD57+NKG2C+ NK cell expansion is associated with reduced leukemia relapse after reduced intensity HCT. Leukemia, 30(2), 456-463.
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