Rnai lentivirus particles

Manufactured by Genechem

Sourced in China

RNAi lentivirus particles are a laboratory tool used for gene silencing experiments. They contain short hairpin RNA (shRNA) sequences that target specific mRNA transcripts, leading to their degradation or translational repression. These particles can be used to stably knock down gene expression in a variety of cell types.

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Lab products found in correlation

Shgli1, by Genechem (1 mentions) Sh con, by Genechem (1 mentions)

2 protocols using rnai lentivirus particles

Lentiviral-mediated RNAi knockdown

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The target sequences of the siRNAs for mouse Tg737and Kif3a and human Tg737 and KIF3A, as well as the randomly aligned sequence used as a negative control, are listed in Table S4. The FG12 lentiviral vector was used to produce double-stranded siRNAs. This vector system and its use have been described previously (Deng et al., 2007 (link)). The RNAi lentivirus particles used to knock down the expression of β-catenin were purchased from GeneChem (China). Cells were incubated with the lentivirus particles for 24 h and then sorted via flow cytometry using GFP as a marker.

Deng Y.Z., Cai Z., Shi S., Jiang H., Shang Y.R., Ma N., Wang J.J., Guan D.X., Chen T.W., Rong Y.F., Qian Z.Y., Zhang E.B., Feng D., Zhou Q.L., Du Y.N., Liu D.P., Huang X.X., Liu L.M., Chin E., Li D.S., Wang X.F., Zhang X.L, & Xie D. (2018). Cilia loss sensitizes cells to transformation by activating the mevalonate pathway. The Journal of Experimental Medicine, 215(1), 177-195.

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Lentiviral Knockdown of WW45 and Gli1

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RNAi lenti-virus particles (sh-con, sh-WW45 and sh-Gli1) were from GeneChem (China). Cells were infected with the indicated lenti-virus particles of the same MOI for 24 hours and then stable knock-down cells were selected with the medium containing puromycin for at least a week.

Li X., Zhou X., Fan Y., Zhang Y., Zu L., Yao F, & Zhou Q. (2016). WW45, a Gli1 binding protein, negatively regulated Hedgehog signaling in lung cancer. Oncotarget, 7(42), 68966-68975.

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