Mouse anti sdha

Chemicals were purchased from Sigma-Aldrich (Deisenhofen, Germany) except Mdivi-1 (Enzo Life Science, Lörrach, Germany) as well as doxycycline, blasticidin and zeocin (Invivogen, San Diego, CA, USA). Cell culture materials and media were obtained from Biochrom (Berlin, Germany). MitoTracker Green^FM (MTG) and MitoSOX™ Red superoxide indicator (MitoSOX) were purchased from Molecular Probes (Eugene, USA).
The following primary antibodies were used: rabbit anti-DNP (Sigma-Aldrich, Deisenhofen, Germany), rabbit anti-PINK1 (Cell Signaling, Boston, MA, USA), mouse anti-β-actin (Cell Signaling, Boston, USA), rabbit anti-COX IV (Cell Signaling, Boston, MA, USA), rabbit anti-Lon protease (Abcam, Cambridge, UK), mouse anti-GAPDH (Abcam, Cambridge, UK), rabbit anti-Ki-67 (Abcam, Cambridge, UK), mouse anti-CDKN2A/p16INK4α (Abcam, Cambridge, UK), rabbit anti-p21 Waf1/Cip1 (Cell Signaling, Boston, MA, USA), mouse anti-MT-CO1 (Abcam, Cambridge, UK), mouse anti-SDHA (Abcam, Cambridge, UK), mouse anti-VDAC (Abcam, Cambridge, UK) and rabbit anti-Fis1 (Cell Signaling, Boston, MA, USA). Secondary antibodies used for immunoblotting were purchased from LI-COR Biosciences (Lincoln, AL, USA). The FITC-labeled antibody for immunofluorescence was purchased from Invitrogen (Carlsbad, CA, USA).

Whole-cell lysates were prepared using lysis buffer (Pierce™ RIPA buffer, Thermo Scientific) supplemented with complete EASYpack Mini Protease Inhibitor Cocktail and PhosSTOP Phosphatase Inhibitor (both from Roche Applied Science). Cell lysates were separated by SDS-gel electrophoresis and transferred to PVDF membranes (Merck Millipore). Immunoblotting was performed using the following antibodies from Cell signaling: mouse anti-β-actin, goat-anti-rabbit (Horseradish peroxidase-conjugated), and anti-mouse (Horseradish peroxidase-conjugated). The following antibodies were purchased from Abcam: mouse anti-Sdha, rabbit anti-ND1, and mouse anti-COX1. Rabbit anti-AMPKα (23A3) and rabbit anti-phospho-AMPKα (Thr172) were purchased from Cell Signaling Technology, and rabbit anti-HIF-1α was from Novus biological.