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2 protocols using fitc rat igg2a κ isotype control

1

Characterization of Immune Cell Phenotypes

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FITC mouse IgG1 κ isotype control (400110), PerCP-Cy5.5 mouse IgG1 κ isotype control (400149), PE mouse IgG1 κ isotype control (400114), PerCP-Cy5.5 anti-human CD3 (300430), FITC anti-human CD8a (300906), PE anti-human CD45 (368510), PerCP-Cy5.5 anti-human IFN-γ (506528), FITC Rat IgG2a κ isotype control (400506), PerCP-Cy5.5 Rat IgG1 κ isotype control (400426), FITC anti-mouse CD3 (100204), FITC anti-mouse CD8a (100705), PE anti-mouse CD45 (103106), and PerCP-Cy5.5 anti-mouse IFN-γ (505822) were purchased from Biolegend. PE anti-human PD-L1 (557924), FITC anti-human PD-L1 (558065), FITC anti-human HLA-A2 (343303), FITC Rat IgG2b κ isotype control (400605), PE Rat IgG2a λ isotype control (400635), and PE anti-mouse PD-L1 (558091) were obtained from BD Biosciences.
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2

Immunoblotting and Flow Cytometry Antibodies

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The following antibodies were used in immunoblotting: rabbit anti-B7-H3 (#14058), rabbit anti-PD-L1 (#13684), rabbit anti-CD86 (#91882), rabbit anti-CD47 (#63000), rabbit anti-Phospho-Stat1 (Tyr701) (#9167), rabbit anti-Phospho-Stat1 (Ser727) (#8826) and rabbit anti-Phospho-Stat3 (Tyr705) (#9145) were purchased from Cell Signaling Technology (Boston, USA). Rabbit anti-Stat1 (10144-2-AP), rabbit anti-Stat3 (10253-2-AP), rabbit anti-HuR (11910-1-AP), rabbit anti-HMGCR (13533-1-AP) and rabbit anti-TTP (12737-1-AP) were from Proteintech (Chicago, USA). Mouse anti-Galectin-9 (ab153673, abcam, Cambridge, UK), rabbit anti-GAPDH (db106, Diagbio, Hangzhou, China), goat anti-mouse PD-L1 (AF1019, R&D systems, Minnesota, USA). The antibodies for flow cytometry: FITC Rat IgG2b κ isotype control (#400605), FITC Rat IgG2a κ isotype control (#400506), PE Rat IgG2a λ isotype control (#400635), PE anti-mouse PD-L1 (#124307), FITC anti-mouse CD3 (#100204), FITC anti-mouse CD8a (#100705) and PE anti-mouse CD45 (#103106) were form Biolegend (Chicago, USA).
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