Rats were randomly assigned to one of four treatment groups: 0 (1X PBS [Mediatech, Inc., Manassas, VA]), 10, 50, or 100μg FLT3L (CDX-301, Celldex Therapeutics, Phillipsburg, NJ). PBS and FLT3L stock endotoxin levels were <0.100 EU/mL (below detectable limits) as assessed by the EndoSafe PTS (Charles River). Animals were injected intraperitoneally once daily for ten days then sacrificed on the eleventh day for collection of the liver, spleen, and mesenteric lymph nodes for analysis (Fig 1 ).
Phosphate buffered saline (pbs)
Manufactured by Charles River Laboratories
PBS (Phosphate-Buffered Saline) is a commonly used buffer solution that maintains a stable pH and osmolarity. It is a versatile reagent used in various laboratory applications, including cell culture, immunoassays, and molecular biology procedures.
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Lab products found in correlation
2 protocols using phosphate buffered saline (pbs)
1
FLT3L Treatment Effects on Immune Cells
2
Subcutaneous Tumor Xenograft Assay
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Housing and care of the animals was approved by the Texas Tech University Institutional Animal Care and Use Committee in accordance with the NIH Guidelines for the Care and Use of Laboratory Animals. Tumor xenograft experiments were performed using H1650 and H1650shTw cells. A total of 5 × 105 cells in 100 μL of PBS were inoculated subcutaneously into the flank of female athymic nude mice at 7–8 weeks of age (Charles River Laboratory, Wilmington, MA). Tumor size was assessed by external measurement of the length (L) and width (W) of the tumor using a digital caliper (Thermo Fisher Scientific). Tumor volume (TV, mm3) was calculated using the following equation: TV = (L × W2)/2.
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