Ros kit

The cellular ROS production of treated A549 and NCI-H157 cells was detected using a ROS Kit (CA1410, Solarbio Life Science, Beijing, China). In brief, treated NSCLC cells were cultured at 37°C for 24 h; after which, they were collected and re-suspended in serum-free culture medium containing 10 µmol/L DCFH-DA at a concentration of 2×10⁷ cells/mL. After incubation at 37°C for 20 min, the NSCLC cells were observed with a laser scanning confocal microscope (Leica, Heidelberg, Germany).

Dendrobine (purity ≥98%) was obtained from Shanghai yuanye Bio-Technology Co., Ltd (Shanghai, China). Two endophytic strains were isolated from Dendrobium in our previous studies and named as Pseudomonas protegens CM-YJ44 (National Center for Biotechnology Information NCBI accession No.MZ674076), Priestia megaterium D-HT207 (NCBI accession No.MK389456), respectively. The dried extract of CM-YJ44 and D-HT207 was collected following the method by Wang et al. [13 ]. The extracts were redissolved in 1 mL Dimethyl Sulfoxide (DMSO) for the following experiments.
The human neuroblastoma SH-SY5Y cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Beijing, China). Dulbecco's modified Eagle's minimum (DMEM) was purchased from Thermo Scientific; fetal bovine serum (FBS) from Gibco; cell Counting Kit-8 (CCK-8) test, ROS kit, MDA kit, GSH kit, CAT kit from Solarbio; the 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolyl-carbocyanine iodide (JC-1) fluorescent probe, SOD kit, NO kit and LDH kit from Beyotime; annexin V-FITC Apoptosis Detection Kit from BD; prime Script RT reagent Kit, SYBR Premix Ex Taq from TaKaRa.