Ab97508

Manufactured by Santa Cruz Biotechnology

Ab97508 is a lab equipment product offered by Santa Cruz Biotechnology. It is a device used for scientific research applications. The core function of Ab97508 is to facilitate the analysis and manipulation of biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Mnase, by New England Biolabs (1 mentions) Suz12, by Merck Group (1 mentions) H 300, by Santa Cruz Biotechnology (1 mentions) Trf 78, by Abcam (1 mentions) Pg m3, by Santa Cruz Biotechnology (1 mentions)

2 protocols using ab97508

Chromatin Immunoprecipitation of Epigenetic Regulators

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

MNase IP was performed as previously described (Sun et al., 2018 (link)) with the following modifications. Cells were lysed in PBS with 0.1% Triton X-100 for nuclei isolation and 1 ul MNase (NEB) per 10 million cells were used for chromatin digestion. Nuclei were pelleted and the mononucleosome-enriched supernatant was quantified spectroscopically (Absorbance A₂₆₀). Equal amount of chromatin from each cell line was used for IP. IP was performed with antibodies followed by incubation with protein A/G beads (Pierce). IP was washed three times with Buffer G 150 with 0.01% NP-40. Membranes were incubated with antibodies against ATRX (Abcam ab97508), DAXX (Santa Cruz sc-7152), EZH2 (Cell Signaling 5246), and SUZ12 (Millipore 07-379).

Qadeer Z.A., Valle-Garcia D., Hasson D., Sun Z., Cook A., Nguyen C., Soriano A., Ma A., Griffiths L.M., Zeineldin M., Filipescu D., Jubierre L., Chowdhury A., Deevy O., Chen X., Finkelstein D.B., Bahrami A., Stewart E., Federico S., Gallego S., Dekio F., Fowkes M., Meni D., Maris J.M., Weiss W.A., Roberts S.S., Cheung N.K., Jin J., Segura M.F., Dyer M.A, & Bernstein E. (2019). ATRX in-frame fusion neuroblastoma is sensitive to EZH2 inhibition via modulation of neuronal gene signatures. Cancer cell, 36(5), 512-527.e9.

+ Open protocol

+ Expand

Immunofluorescence Analysis of Telomere-Associated Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies were TRF-2 (SantaCruz H-300, sc-9143, rabbit polyclonal, 20 µg/ml), γH2AX (Abcam, Anti-gamma H2A.X (phospho S139 antibody [9F3], ab26350, mouse monoclonal, 10 µg/ml), PML (PG-M3, SantaCruz, sc-966, mouse monoclonal, 2 µg/ml), anti-TRF1 (Abcam, TRF-78, ab10579), TIN2 (Abcam, ab197894), POT1 (Abcam, ab90552), RAP1 (Abcam, ab40144), ATRX (Abcam, ab97508), TopoIIIα (SantaCruz, sc13060, rabbit polyclonal), DAXX (Abcam, ab32140), LINE-1 (SantaCruz H110, sc67197, rabbit polyclonal), LINE-1 (chA1-L1-RNP, mouse monoclonal, 40 µg/ml) [46] , or β-Tubulin (9F3) rabbit IgG mAb (Cell Signaling, Cat. # 2128S, 1:10000, overnight, 4 °C), Anti-p21 antibody [EPR18021] (Abcam, ab188224), hTERT-antibody (Abcam, ab183105), Anti-Caspase-3 (Abcam, ab13847).

Aschacher T., Wolf B., Aschacher O., Enzmann F., Laszlo V., Messner B., Türkcan A., Weis S., Spiegl-Kreinecker S., Holzmann K., Laufer G., Ehrlich M, & Bergmann M. (2019). Long interspersed element-1 ribonucleoprotein particles protect telomeric ends in alternative lengthening of telomeres dependent cells. Neoplasia (New York, N.Y.), 22(2), 61-75.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!