λ dna hindiii
λ DNA/HindIII is a DNA ladder product offered by New England Biolabs. It consists of fragments of λ DNA digested with the HindIII restriction enzyme, providing a set of DNA fragments of known sizes for use as molecular weight markers in gel electrophoresis.
Lab products found in correlation
2 protocols using λ dna hindiii
DNA Samples for Biopolymer Translocation
Plasmid DNA Isolation and Cloning
PCRs were performed as previously described (11 (link)). The sequences for all primers used in this study are listed in
Cloning was performed as previously described (11 (link)), with minor modifications. All ligations were performed at a 3:1 M ratio of insert to vector with 40 U of T4 DNA ligase (New England Biolabs) in a final concentration of 1× ligase buffer. Chemically competent E. coli DH5α cells were prepared using a CaCl2 method, and transformation was performed via heat shock.
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