The RL95 to 2 (No.TCHu198), Ishikawa, HEC-1-A(No.TCHu149) and HEC-1-B(No.TCHu115) EC cell lines were purchased from Shanghai Chinese Academy of Sciences Cell Bank and were cultured in DMEM, containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.；Cat：10099141C) and 1% penicillin/streptomycin (Gibco; Thermo Fisher Scientific, Inc.; Cat: 15140148) at 37 °C in a humidified incubator with 5% CO2. The RL95 to 2 and HEC-1-A cell lines were transfected with negative control (NC) mimic, miR-133b mimic, NC inhibitor or miR-133b inhibitor (all cell lines were purchased from Guangzhou RiboBio Co., Ltd). Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.; Cat: 11668019), was used for transfection, according to the manufacturer's instructions and subsequent experimentation was performed 48 h following.
Mir 133b inhibitor
Manufactured by RiboBio
Sourced in China
The MiR-133b inhibitor is a laboratory reagent used in research applications. It functions to inhibit the expression or activity of the microRNA miR-133b. miR-133b is involved in various cellular processes and its regulation can be studied using this inhibitor. The core function of this product is to provide researchers with a tool to modulate miR-133b levels in experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Mir 133b mimic, by RiboBio (2 mentions)
Mimic nc, by RiboBio (1 mentions)
Inhibitor nc, by RiboBio (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Igf 1, by Thermo Fisher Scientific (1 mentions)
Ovoil, by Vitrolife (1 mentions)
G2 plus, by Vitrolife (1 mentions)
2 protocols using mir 133b inhibitor
1
Transfection of Endometrial Cancer Cell Lines
2
IGF-1 Effects on Isolated MI Oocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The isolated MI oocytes were cultured at 37°C and 5% CO2 in G-2 PLUS (Vitrolife, Frölunda, Sweden) treated with (45 oocytes) or without (62 oocytes) 200 ng/ml IGF-1 (Peprotech, Rocky Hill, NJ, USA) for 24 h, then covered with ovoil (Vitrolife). After 24 h, all oocytes were stored at 80°C until RNA was extracted. The average age of patients without IGF-1 treatment was 31.66±3.90. The average age of patients with IGF-1 treatment was 31.25±3.31. No apparent bias was detected between these two groups.
The 293T cells injected with MiR-133b mimic, MiR-133b mimic negative control, MiR-133b inhibitor, or MiR-133b inhibitor negative control (NC) (Ribobio, Guangzhou, China) were cultured at 37°C and 5% CO2 in DMEM (Gibco, Grand Island, USA) and supplemented with 10% fetal bovine serum and 1% antibiotics (100 U/ml penicillin and 100 U/ml streptomycin.
The 293T cells injected with MiR-133b mimic, MiR-133b mimic negative control, MiR-133b inhibitor, or MiR-133b inhibitor negative control (NC) (Ribobio, Guangzhou, China) were cultured at 37°C and 5% CO2 in DMEM (Gibco, Grand Island, USA) and supplemented with 10% fetal bovine serum and 1% antibiotics (100 U/ml penicillin and 100 U/ml streptomycin.
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